A recent issue of ACS Chemical Biology includes some 25 articles on various aspects of CRISPR/Cas systems – mechanisms and applications. While not all of the articles will be of direct interest to insect biologists, many are and anyone working with these systems or planning on working with these systems will benefit from spending some time with this issue.
The paper by Mir et al. Type II-C CRISPR-Cas9 Biology, Mechanism, and Application is a good place to start if one is interested in an overview of CRISPR/Cas9 systems. Likewise the paper by Pyzocha and Chen, Diverse Class 2 CRISPR-Cas Effector Proteins for Genome Engineering Applications
provides a review of CRISPR systems with single effector proteins – such as the Cas9 system. But there are other single effector CRISPR systems – Cpf1 for DNA editing, Cas13a and Cas13b for RNA editing.
While this entire issue makes for great reading, some of the other papers in this issue that might be of particular interest to insect biologists are
Thompson et al’s The Future of Multiplexed Eukaryotic Genome Engineering;
Burt and Crisanti’s Gene Drive: Evolved and Synthetic;
Wilson and Gilbert’s The Promise and Challenge of In Vivo Delivery for Genome Therapeutics;
Komor et al’s Editing the Genome Without Double-Stranded DNA Breaks;
Pawelczak et al’s Modulating DNA Repair Pathways to Improve Precision Genome Engineering;
Bondy-Denomy‘s Protein Inhibitors of CRISPR-Cas9;
Marshall and Akbari’s Can CRISPR-Based Gene Drive Be Confined in the Wild? A Question for Molecular and Population Biology; and
Rose et al’s Rheostatic Control of Cas9-Mediated DNA Double Strand Break (DSB) Generation and Genome Editing
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Special Issue on the Chemical Biology of CRISPR
Alyson G. Weidmann and Amit ChoudharyACS Chemical Biology 2018 13 (2), 283-284 -
Introducing Our Authors
Brian WylerACS Chemical Biology 2018 13 (2), 285-289 -
Spotlight: A Conversation with Laura Kiessling and Jennifer Doudna
Laura L. Kiessling and Jennifer A. DoudnaACS Chemical Biology 2018 13 (2), 290-295 -
Frontiers in CRISPR
Alyson G. WeidmannACS Chemical Biology 2018 13 (2), 296-304 -
Discovering the Genome-Wide Activity of CRISPR-Cas Nucleases
Shengdar Q. TsaiACS Chemical Biology 2018 13 (2), 305-308 -
Discovery of Oligonucleotide Signaling Mediated by CRISPR-Associated Polymerases Solves Two Puzzles but Leaves an Enigma
Eugene V. Koonin and Kira S. MakarovaACS Chemical Biology 2018 13 (2), 309-312 -
The Future of Multiplexed Eukaryotic Genome Engineering
David B. Thompson, Soufiane Aboulhouda, Eriona Hysolli, Cory J. Smith, Stan Wang, Oscar Castanon, and George M. ChurchACS Chemical Biology 2018 13 (2), 313-325 -
Identifying Novel Enhancer Elements with CRISPR-Based Screens
Jason C. Klein, Wei Chen, Molly Gasperini, and Jay ShendureACS Chemical Biology 2018 13 (2), 326-332 -
Genome Editing: Insights from Chemical Biology to Support Safe and Transformative Therapeutic Applications
Renee D. Wegrzyn, Andrew H. Lee, Amy L. Jenkins, Colby D. Stoddard, and Anne E. CheeverACS Chemical Biology 2018 13 (2), 333-342 -
Gene Drive: Evolved and Synthetic
Austin Burt and Andrea CrisantiACS Chemical Biology 2018 13 (2), 343-346 -
Diverse Class 2 CRISPR-Cas Effector Proteins for Genome Engineering Applications
Neena K. Pyzocha and Sidi ChenACS Chemical Biology 2018 13 (2), 347-356 -
Type II-C CRISPR-Cas9 Biology, Mechanism, and Application
Aamir Mir, Alireza Edraki, Jooyoung Lee, and Erik J. SontheimerACS Chemical Biology 2018 13 (2), 357-365 -
CRISPR Approaches to Small Molecule Target Identification
Marco Jost and Jonathan S. WeissmanACS Chemical Biology 2018 13 (2), 366-375 -
The Promise and Challenge of In Vivo Delivery for Genome Therapeutics
Ross C. Wilson and Luke A. GilbertACS Chemical Biology 2018 13 (2), 376-382 -
Editing the Genome Without Double-Stranded DNA Breaks
Alexis C. Komor, Ahmed H. Badran, and David R. LiuACS Chemical Biology 2018 13 (2), 383-388 -
Modulating DNA Repair Pathways to Improve Precision Genome Engineering
Katherine S. Pawelczak, Navnath S. Gavande, Pamela S. VanderVere-Carozza, and John J. TurchiACS Chemical Biology 2018 13 (2), 389-396 -
Repair of a Site-Specific DNA Cleavage: Old-School Lessons for Cas9-Mediated Gene Editing
Danielle N. Gallagher and James E. HaberACS Chemical Biology 2018 13 (2), 397-405 -
CRISPRi and CRISPRa Screens in Mammalian Cells for Precision Biology and Medicine
Martin KampmannACS Chemical Biology 2018 13 (2), 406-416 -
Protein Inhibitors of CRISPR-Cas9
Joseph Bondy-DenomyACS Chemical Biology 2018 13 (2), 417-423 -
Can CRISPR-Based Gene Drive Be Confined in the Wild? A Question for Molecular and Population Biology
John M. Marshall and Omar S. AkbariACS Chemical Biology 2018 13 (2), 424-430 -
Multidimensional Control of Cas9 by Evolved RNA Polymerase-Based Biosensors
Jinyue Pu, Kaitlin Kentala, and Bryan C. DickinsonACS Chemical Biology 2018 13 (2), 431-437 -
Rheostatic Control of Cas9-Mediated DNA Double Strand Break (DSB) Generation and Genome Editing
John C. Rose, Jason J. Stephany, Cindy T. Wei, Douglas M. Fowler, and Dustin J. MalyACS Chemical Biology 2018 13 (2), 438-442 -
A Single-Chain Photoswitchable CRISPR-Cas9 Architecture for Light-Inducible Gene Editing and Transcription
Xin X. Zhou, Xinzhi Zou, Hokyung K. Chung, Yuchen Gao, Yanxia Liu, Lei S. Qi, and Michael Z. LinACS Chemical Biology 2018 13 (2), 443-448 -
Generation of Optogenetically Modified Adenovirus Vector for Spatiotemporally Controllable Gene Therapy
Kazuo Takayama and Hiroyuki MizuguchiACS Chemical Biology 2018 13 (2), 449-454 -
Chemical Control of a CRISPR-Cas9 Acetyltransferase
Jonathan H. Shrimp, Carissa Grose, Stephanie R. T. Widmeyer, Abigail L. Thorpe, Ajit Jadhav, and Jordan L. MeierACS Chemical Biology 2018 13 (2), 455-460 -
A Cleavage-Responsive Stem-Loop Hairpin for Assaying Guide RNA Activity
Tara R. deBoer, Noreen Wauford, Jing-Yi Chung, Miguel Salvador Torres Perez, and Niren MurthyACS Chemical Biology 2018 13 (2), 461-466 -
CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide
Marie K. Schwinn, Thomas Machleidt, Kris Zimmerman, Christopher T. Eggers, Andrew S. Dixon, Robin Hurst, Mary P. Hall, Lance P. Encell, Brock F. Binkowski, and Keith V. WoodACS Chemical Biology 2018 13 (2), 467-474 -
Two-Color 810 nm STED Nanoscopy of Living Cells with Endogenous SNAP-Tagged Fusion Proteins
Alexey N. Butkevich, Haisen Ta, Michael Ratz, Stefan Stoldt, Stefan Jakobs, Vladimir N. Belov, and Stefan W. HellACS Chemical Biology 2018 13 (2), 475-480 -
Conformational Dynamics of DNA Binding and Cas3 Recruitment by the CRISPR RNA-Guided Cascade Complex
Paul B. G. van Erp, Angela Patterson, Ravi Kant, Luke Berry, Sarah M. Golden, Brittney L. Forsman, Joshua Carter, Ryan N. Jackson, Brian Bothner, and Blake WiedenheftACS Chemical Biology 2018 13 (2), 481-490
