A recent publication by Strecker et al., 2019, opens up the intriguing possibility that some transposons may be able to be guided to specific sites in the genome thereby overcoming perhaps the greatest hurdle that prevents them from being used […]
transposase
Improved Honeybee Germline Transformation
Otte et al (2018) build upon their previous work (Schulte et al. 2014) in this new publication and demonstrate a twofold improved efficiency at generating transgenic honeybees using the piggyBac transposon system. The inability to generate high transformation rates has […]
Active Recombinant Tol2 Transposase
Ni et al (2016) report successfully expressing and purifying Tol2 transposase and demonstrate its activity in vitro and in vivo. Although they were working with zebrafish the system they describe might be of some interest to insect biologists. Tol2 is […]
Transgenic Honeybees – Finally!
Genome modification technologies have been increasing steadily in recent years. With the exception of direct genome editing technologies (ZFNs, TALENs and CRISPRs) variously technologies for use in insects rely heavily on transposon platforms to move these technologies into target genomes. […]