transgenics

Finding Enhancers in Mosquitoes and Beyond

This post was written by Joseph Sarro and Keshava Mysore In a recent paper published in BMC Genomics, Behura and Sarro et al. (2016) report on the use of FAIRE-seq, formaldehyde-assisted isolation of regulatory elements paired with DNA sequencing, for […]

Powerful Genetic and Genomic Toolbox of Nasonia Wasps

Jeremy Lynch (2015) has published a nice overview of the genetic/genomic tools currently available for Nasonia species. Insects in the order of Hymenoptera are emerging as new genetic and genomic tools for evolutionary biology, development, and behavioral ecology. Among over […]

More Mosquito Genome Editing – With Instructions

Kistler et al. have just published the results of their extensive use of CRISPR/Cas9-based knock-out and knock-in technology in Aedes aegypti in Cell Reports and anyone considering using these technologies in insects will find this paper of interest. The authors […]

New ΦC31 Resources

A rapid method for generating and finding ΦC31 landing sites (attP) with the desired expression characteristics as well as a new method for creating transgene arrays in vivo was recently described by Knapp et al (2015). Albeit these methods were […]

International Short Course on Insect Transgenesis: Shanghai, China March 16-20, 2015

International Short Course on Insect Transgenesis. (download poster) Applications are now being accepted. The workshop will provide a small number (~15) of rigorously-selected graduate students, postdocs and early-stage faculty an opportunity to learn how to plan and carry out transgenesis […]

A Collection of Methods for Editing Insect Genomes

The August 2014 issue of METHODS is devoted to the topic of “Editing and Investigating Genomes with TALE and CRISPER/Cas Systems: Genome Engineering Across Species Using TALENS” Five of the 14 protocols included in this issue are specifically focused on […]

Transgenic TALEN Expression to Control Sex in Bombyx

Analysis of gene function in insects by editing the genome, or reverse genetics, is now greatly facilitated thanks to powerful genetic technologies, such as TALENs, ZFNs, and more recently CRISPR/Cas9, becoming available in many organisms. However, screening of transgenic mutated […]