ReMOT Control Gene Editing is Expanded to Anopheles stephensi

Traditional genome editing in insects involves directly injecting embryos.  However, most insect embryos are small and sensitive to manipulation.  This makes germ-line modifications difficult to achieve.  Therefore, the recent development of a new gene editing approach based on injecting adult mosquitoes to target oocytes is a major advance.

“Sexing” Technologies – A Review

In a recent review,  Lutrat et al. (2019) provide a comprehensive summary of current (since 2003) sexing methods available for various dipteran pest species. The review distinguishes between early and late acting sexing methods and elaborates on the techniques used […]

Heritable Gene Editing in Thermobia domestica

Ohde et al report the first successful development and use of CRISPR/Cas9 in the basally branching insect Thermobia domestica. By co-injecting Cas9 nuclease with sgRNA targeting a T. domestica ortholog of the Drosophila melanogaster white gene (Td-w), the authors created […]

2019 IGTRCN Technical Short Course – Applications Open

2019 IGTRCN Technical Short Course on Insect Genetic Technologies: Theory and Practice July 21 – 26, 2019 The Insect Transformation Facility Institute for Bioscience and Biotechnology Research University of Maryland College Park Applications by April 1, 2019 (24 seats available) […]

Enhancer Trapping in Mosquitoes

A recent manuscript published on Genes Genomes Genetics by Reid et al (2018) reports an exciting improvement to piggyBac-based promoter-trap designed in Anopheles stephensi. The development of this new piggyBac-based promoter-trap designs provides further knowledge and exciting venues to examine […]

Microinjection of the Sand Fly Lutzomyia longipalpis, a Vector for Leishmaniasis

Two recent papers, Jefferies et al (2018) and Martin-Martin et. al (2018), describe experiments to develop and optimise a sand fly microinjection protocol. There are 2 authors to this post: Alla Madich, Simon Collier, technical constraints on this website allow […]

One-step recombination-mediated cassette exchange (RMCE) in Drosophila suzukii

Schetelig et al. (2018) describe a relatively efficient recombinase-mediated cassette exchange (RMCE) system for engineering the recently sequenced genome (Chiu et al., 2013) of the spotted wing drosophila, Drosophila suzukii, which is a significant pest of small and stone fruits. […]

Whole-brain, Single Cell Activity Mapping in Transgenic Gryllus

Watanabe et al in eNeuro (2018) report their successful development of transgenic cricket Gryllus bimaculatus that bears a transcriptional reporter for whole-brain, single cell-resolution activity mapping. In order to construct a transcriptional reporter for activity mapping, it is critical to […]

ReMOT control delivery technology holds promise for CRISPR-Cas9 activity independent of embryonic injection

A new report in Nature Communications by Chaverra-Rodriguez et al. details a novel Cas9-delivery approach for insect genetic modification. In their work, Chaverra-Rodriguez et al introduce a technology they term “ReMOT control”, or Receptor-Mediated Ovary Transduction of Cargo. In their […]