transgenic

ReMOT control delivery technology holds promise for CRISPR-Cas9 activity independent of embryonic injection

A new report in Nature Communications by Chaverra-Rodriguez et al. details a novel Cas9-delivery approach for insect genetic modification. In their work, Chaverra-Rodriguez et al introduce a technology they term “ReMOT control”, or Receptor-Mediated Ovary Transduction of Cargo. In their […]

IGTRCN 2018 Technical Workshop – July 22-27

2018 IGTRCN Technical Short Course on Insect Genetic Technologies: Theory and Practice July 22 – 27, 2018 The Insect Transformation Facility Institute for Bioscience and Biotechnology Research University of Maryland College Park Applications by April 2, 2018 (24 seats available) […]

Promoters From Two Sap-Sucking Pests

Qian et al (2016) using bioinformatic and transient heterologous expression systems have identified three strong promoters from the genomes of the Hemipterans  Nilaparvata lugens and Nephotettix cincticeps. The abundance of genome sequence data is a boon to insect science and […]

Animal and Insect Transgenics – Parallels

James Murray and Elizabeth Maga recently recounted briefly the development and evolution of animal transgenic technologies in a recent issue of Transgenic Research and the similarities and differences between the development and evolution of insect transgenic technologies, which were ongoing […]

Short Course on Insect Genetic Technologies – August 2015

The Insect Genetic Technologies Research Coordination Network is offering a short course on insect genetic technologies August 17-21, 2015. Today there are myriad technologies for modifying genomes. Technologies are emerging rapidly and it is a challenge for investigators to keep […]

Anopheles gambiae Transgenesis Tool Box – Major Additions

Volohonsky et. al. (2015) in a just-published paper in G3 entitled  “Tools for Anopheles gambiae transgenesis”  describe a wealth of new tools for creating transgenic An. gambiae. There has been a protocol for Anopheles gambiae transgenesis for over 15 years, […]

Electroporation of Horn Fly Embryos

Qiang Xu, et al. 2015 describe their efforts to transform the horn fly, Haematobia irritans, by elctroporation. Transgenic technology has been considered an effective technique for the construction of genetically-modified insects for the purposes of insect control. Microinjection with piggyBac transposable […]

Fly Facility, University of Cambridge, UK

Insect Genetic Technologies Service Provider – Drosophila – Non-commercial Fly Facility, University of Cambridge, UK – http://www.flyfacility.gen.cam.ac.uk Our Drosophila microinjection service is available to fly researchers throughout the European Union. We can inject flies (including non-melanogaster Drosophila species) for P-element/piggyback […]

IGTRCN Trainees – Fall 2014!

The IGTRCN is working to diffuse knowledge and technology from those who ‘know’ and ‘have’ to those who do not but need to. The Peer-to-Peer Training Fellowships are intended to facilitate the process by providing modest resources to encourage interactions. […]

“Trojan Horse” Peptides: Delivery Systems With Diverse Applications

Everyone wants to get things into cells. Biomedical researchers, clinicians and even insect biologists. Consequently there is a large research community – primarily in the realm of biomedicine – that is actively exploring “delivery systems”. Cell Penetrating Peptides (CPPs), sometimes […]

Medusa: Harnessing a Sex-Linked Transgenic Drive System to Control Insect Populations

Segregation distorters (also known as drive genes) are selfish genetic elements with a natural fitness advantage – they are passed to greater than fifty percent of offspring in the next generation, breaking Mendel’s first law of inheritance. In nature, drive […]

Insect Genetic Technologies Service Providers

Insect Genetic Technology Service Providers If you would like to add to this list use the “SUBMISSIONS” button and select “Knowledgebase” For insects other than Drosophila melanogaster Non-Commercial The Institute for Bioscience and Biotechnology Insect Transformation Facility, University of Maryland […]

The Road Ahead for Using Gene Drive Systems

Emerging technologies have employed chemical, ecological and genetic strategies for effective and economical pest management. The various strategies around the transgenic augmentation of pests has shown great promise in being able to combat vector borne diseases and eradicate agricultural pests […]

WHO’s Guidance on Testing Genetically Modified Mosquitoes

The World Health Organization (WHO)  has published a guidance document that provides a framework by which genetically modified mosquitoes might be developed and tested as tools for controlling mosquito populations and/or pathogen transmission. This guidance comes at a time when […]

Six Tandem Cherries -> Very Bright Insects

Insect genetic modification technologies rely heavily on the use of fluorescent proteins as genetic markers, expression reporters etc. Colors vary, as do fluorescent outputs. Shearin et al. (2014) describe their efforts to increase signals from two popular fluorescent proteins, EGFP […]

Delivering Reprogramed Meganucleases With Lentiviruses- Is this Applicable to “Difficult” Insects?

Delivering biologically relevant nucleic acids and proteins to appropriate cells of multicellular organisms for the purposes of genetically modifying either somatic or germline genomes is a central problem for not only insect biologists but for those working on other organisms […]

Genetically Modified Annelids: Platynerieis dumerilii. Not Unlike Modifying Insects

The bristle worm, Platynerieis dumerilii, is a marine annelid with a biology that makes it of interest because of the development of its nervous system, reproductive cycles that are synchronized with lunar phases and its neuroendocrinology among other things. Even […]

Transgenic Honeybees – Finally!

Genome modification technologies have been increasing steadily in recent years. With the exception of direct genome editing technologies (ZFNs, TALENs and CRISPRs) variously technologies for use in insects rely heavily on transposon platforms to move these technologies into target genomes. […]

Water Fleas and Insects – Common Challenges for Genetic Modification – Shared Solutions?

The water flea, Daphnia pulex, is a microcrustacean that has become a model for biomedical research and, perhaps more significantly, a model for probing G x E (Genetics x Environment) interactions. Some genetic modification methods have been developed for Daphnia […]

Advanced Mosquito Genetic Engineering

For mosquitoes the number of genetic modification technologies that can and have been applied continues to grow, and while still not as deep as the Drosophila toolbox the mosquito toolbox is still impressive in the right hands. Bernardini et al. […]

Marking Clones: Past and Future

As Perrimon and colleagues point out chimeras or mosaics have played and continue to play an important role in our analysis of development.  In Drosophilia the technology for creating mosaics is mature and powerful.  Even before transgenic technologies provided precision […]