transformation

Delivering gene-editing proteins in vivo using a new strategy.

Delivering genetic technologies to appropriate cells at the appropriate times is, for many insect systems, the biggest obstacle to the widespread use of these technologies. Microinjecting developing embryos with DNA, RNA or protein remains the dominant mode of delivery in […]

Injectoporation for Gene Delivery

Let’s see.  What do we have so far? Great genomic resources?  Check. Great gene vectors?  Check. Great genome modification technologies?  Check. Effective insect genetic technology delivery systems for somatic and germ cells?  Not so much, unfortunately. Gene, transgene and RNA […]

φC31 in Anopheles gambiae: A How-To

In terms of genetic engineering of insects, the current most commonly used technique for germline transformation involves the use of mobile transposable genetic elements, which are simply inefficient, have limited carrying capacities, and integrate randomly into genomes resulting in insertional […]

Robotic Injection of Embryos – Ready for Insects?

  Spaink et al (2013) describe their use of a robotic injection system as a high throughput screening tool in zebra fish, Danio rerio, embryos.  Automated and semi-automated injection systems for zebra fish embryos as well as other systems are […]