transformation

Improved Honeybee Germline Transformation

Otte et al (2018) build upon their previous work (Schulte et al. 2014) in this new publication and demonstrate a twofold improved efficiency at generating transgenic honeybees using the piggyBac transposon system.  The inability to generate high transformation rates has […]

Western Corn Rootworm, Diabrotica virgifera virgifera, germline transformation.

Chu and colleagues in a recently published paper in Insect Molecular Biology describe their successful efforts to deploy transposon-based technologies in the economically important beetle, Diabrotica virgifera virgifera, and it is an interesting case study in how to develop genetic […]

Megaselia and Chironomus Germline Transformation

In a recent paper in Development Genes Evolution., Caroti et al. (2015) reported on their efforts to extend the piggyBac transposon-based germline transformation system to the Diptera Megaselia abdita and Chironomus riparius and to use it for in vivo labelling […]

Short Course on Insect Genetic Technologies – August 2015

The Insect Genetic Technologies Research Coordination Network is offering a short course on insect genetic technologies August 17-21, 2015. Today there are myriad technologies for modifying genomes. Technologies are emerging rapidly and it is a challenge for investigators to keep […]

Electroporation of Horn Fly Embryos

Qiang Xu, et al. 2015 describe their efforts to transform the horn fly, Haematobia irritans, by elctroporation. Transgenic technology has been considered an effective technique for the construction of genetically-modified insects for the purposes of insect control. Microinjection with piggyBac transposable […]