TALEN

Loss of Function Mutations – Approaches, Limitations

Technologies are democratizing insect genetics and it seems as if everyone is thinking about making ‘knockouts’.  This is a good thing but as Housden et al. nicely explain in a recent paper in Nature Reveiws Genetics explain: “although LOF (loss […]

Dopamine receptor knock-outs in crickets

Awata et al. (2015) have used Cas9 to knock-out Dop1 in Gryllus bimaculatus to assess the role of dopamine neurons in mediating reward and punishment. Functional genomics technologies like transposons and first and second generation gene-editing technologies have been available […]

Sawfly and Ladybird Beetle Gene Editing

Hatakeyama et al (2015) have successfully used gene editing technology in Athalia rosae (sawfly) and Harmonia axyridis (ladybird beetle), enlarging the range of insects in which these technologies have been applied.  These results are reported in Insect Molecular Biology. Hatakeyama […]

Compendium of Genome Engineering

Koen Venken and colleagues have just published an excellent review of ‘Genome Engineering: Drosophila melanogaster and beyond‘ that is comprehensive and highly informative, and should serve as a useful reference for anyone interested in genome modification techniques with nucleotide precision […]

Optimized TALEN Application and Screening in Drosophila melanogaster

Lee et al 2015 provide a detailed methodology for gene editing using TALENs in Drosophila melanogaster. While TALEN use is relatively well established in D. melanogaster, fine tuning the injection concentrations of designer nucleases allows for the maximum efficiency in […]

TGV: Programmable DNA Binding Proteins for Live Visualization of Chromatin

Transcription activator-like effector (TALE) technologies are being used in a variety of creative ways by swapping functional protein domains that are connected to the ‘programmed’ DNA binding domain.  Miyanari (2014) and Miyanari et al (2013) describe their use of TALEs […]