site-specific recombination

Chemical and Light Inducible Recombinases

In a recent Nature Communications publication Weinberg et al., (2019) report developing and testing a repertoire of inducible site-specific recombinases capable of being activated by different inducers such as small molecules, light, and temperature.   While developed and tested in mammalian cells and transgenic mice, they will be widely useful in insect systems.

Genetically Modified Plant-Feeding Insects: A DARPA Project

When transgenic mosquito technology was being developed an idea was floated to use genetically modified mosquitoes as flying syringes to deliver important antigens to immunize people against various pathogens.  Informed consent among other things made this an overly complex problem […]

New ΦC31 Resources

A rapid method for generating and finding ΦC31 landing sites (attP) with the desired expression characteristics as well as a new method for creating transgene arrays in vivo was recently described by Knapp et al (2015). Albeit these methods were […]

Advanced Mosquito Genetic Engineering

For mosquitoes the number of genetic modification technologies that can and have been applied continues to grow, and while still not as deep as the Drosophila toolbox the mosquito toolbox is still impressive in the right hands. Bernardini et al. […]

Marking Clones: Past and Future

As Perrimon and colleagues point out chimeras or mosaics have played and continue to play an important role in our analysis of development.  In Drosophilia the technology for creating mosaics is mature and powerful.  Even before transgenic technologies provided precision […]

Improved Lineage Marking in Drosophila

Marking cells has been a powerful tool for developmental biologists studying Drosophila melanogaster for many decades.  Insect genetic technologies, in particular site specific recombination systems, have allowed this lineage marking and tracking method to become highly robust and controlable.  Enhancer […]