SIT

Gene Editing Screwworms and Blowflies

Paolo et al. report the first successful use of CRISPR/Cas9 in the New World Screwworm fly, Cochliomyia hominivorax (C. hominivorax), a parasite of warm blooded animals including humans, as well as a CRISPR/Cas9 protocol for the Australian sheep blowfly, Lucilia […]

“Sexing” Technologies – A Review

In a recent review,  Lutrat et al. (2019) provide a comprehensive summary of current (since 2003) sexing methods available for various dipteran pest species. The review distinguishes between early and late acting sexing methods and elaborates on the techniques used […]

Trojan Female Technique: Mitochondria-based population suppression.

The ‘Trojan Female Technique” is a genetics-based population suppression strategy.  Wolff et al.(2017) report results of empirical tests of the idea using Drosophila melanogaster as a model system and show net population suppression. Genetics-based pest control strategies are becoming increasingly […]

DNA-free genome editing in the agricultural pest Ceratitis capitata

The genome editing revolution creates new opportunities to improve pest management strategies, yet biosafety concerns over off-target effects and the use of foreign DNA present roadblocks to the development of modified organisms destined for field use. In a recent addition […]

Transgenic blow flies for SIT

Yan and Scott have constructed transgenic Lucilia cuprina, Australian sheep blowfly, resulting in conditional lethality of female embryos and their results are reported in Scientific Reports. With an interest in enabling the development of efficient systems for producing male blowflies […]

Two Bombyx Testis-Specific Promoters

The level of sophistication with which one can genetically manipulate insects is very much determined by how well transgene expression can be regulated. Xu et al (2014) in Insect Molecular Biology describe their successful efforts to isolate promoters from two […]

Transgenic TALEN Expression to Control Sex in Bombyx

Analysis of gene function in insects by editing the genome, or reverse genetics, is now greatly facilitated thanks to powerful genetic technologies, such as TALENs, ZFNs, and more recently CRISPR/Cas9, becoming available in many organisms. However, screening of transgenic mutated […]

Insect Genetic Technologies To Control Blow Flies

Blow flies (family Calliphoridae) can be serious and dangerous pests. The new world screwworm fly (Cochliomyia hominivorax) as the latin name says can be a man-eater (hominivorax). While many blow flies prefer to lay eggs on dead and decaying animals, […]