sgRNA

Cas9 Gene Drive, Sex-Conversion and Evolved Resistance

In a recent manuscript, KaramiNejadRanjbar et al. demonstrate the development of a proof of principle Cas9-based suppression gene drive in D. melanogaster that can be applied to pest insects, and discuss the implications of resistance allele formation for practical use […]

Target Site Accessibility and Development of Better Guide RNA Design Tools For CRISPR/Cas9

A recent paper by the Lee et al (2018) has shown that the present CRISPR/Cas9 design tools do not accurately predict the activity of single-gene targeting gRNA sequences. They also report that the non sequence based features like DNA accessibility […]

Intramolecular interactions in gRNA may influence Cas9 cleavage activity

In an article recently published in Nature Communications, Thyme et al (2016) discuss the interactions of the sequences of sgRNAs, the role of chromatin, and how these factors might interfere with Cas9 cleavage activity. By investigating the activity of Cas9 […]

CHOPCHOP v2: Improved online CRISPR/Cas9 target prediction tool

In a paper published in Nucleic Acids Research, Labun et al. (2016), the developers of CHOPCHOP, report on their updated version: CHOPCHOPv2 . The first version of CHOPCHOP was released by a team of researchers from Harvard University a little […]