piggyBac

Improved Honeybee Germline Transformation

Otte et al (2018) build upon their previous work (Schulte et al. 2014) in this new publication and demonstrate a twofold improved efficiency at generating transgenic honeybees using the piggyBac transposon system.  The inability to generate high transformation rates has […]

A Synthetic Biology Approach Confers Resistance to Zika in Ae. aegypti

In a recent PNAS publication by Buchman et al (2019), the authors report on the establishment and validation of transgenic Aedes aegypti containing a synthetic anti-ZIKV cassette, capable of conferring complete refractoriness to the virus. Aided by piggyBac transposon-mediated insertion […]

Enhancer Trapping in Mosquitoes

A recent manuscript published on Genes Genomes Genetics by Reid et al (2018) reports an exciting improvement to piggyBac-based promoter-trap designed in Anopheles stephensi. The development of this new piggyBac-based promoter-trap designs provides further knowledge and exciting venues to examine […]

Whole-brain, Single Cell Activity Mapping in Transgenic Gryllus

Watanabe et al in eNeuro (2018) report their successful development of transgenic cricket Gryllus bimaculatus that bears a transcriptional reporter for whole-brain, single cell-resolution activity mapping. In order to construct a transcriptional reporter for activity mapping, it is critical to […]

HyperActive piggyBac Transposase for Transgenesis

Eckermann et al. (2018) recently published a report in Insect Biochemistry and Molecular Biology demonstrating the efficacy of hyperactive piggyBac transposase for creating transgenic insects, which may be of interest to researchers looking to initiate transgenic projects in ‘non-model’ insect […]

Western Corn Rootworm, Diabrotica virgifera virgifera, germline transformation.

Chu and colleagues in a recently published paper in Insect Molecular Biology describe their successful efforts to deploy transposon-based technologies in the economically important beetle, Diabrotica virgifera virgifera, and it is an interesting case study in how to develop genetic […]

Transgenic blow flies for SIT

Yan and Scott have constructed transgenic Lucilia cuprina, Australian sheep blowfly, resulting in conditional lethality of female embryos and their results are reported in Scientific Reports. With an interest in enabling the development of efficient systems for producing male blowflies […]