NHEJ

CRISPR to go – a guide to gene editing Tephritid flies at remote facilities

Sim et al. (2018) recently published a paper in Insect Molecular Biology describing CRISPR/Cas 9 methods for gene editing in three genera of Tephritid fruit flies: Anastrepha, Bactrocera, and Ceratitis. CRISPR/Cas9 allows for targeted gene editing through homology driven repair […]

Strategies for Driving Out Invasive Species

Prowse et al. (2017) present their modelling work in the Proceedings of The Royal Society B on various different CRISPR/Cas9-based gene drives aimed at suppressing populations of exotic vertebrate species on islands (focusing on mice (Mus musculus)).  The issues addressed […]

Cas9 Expression Patterns Impact on Resistance Evolution in Different Genetic Backgrounds

Gene drive systems are sensitive to the evolution of resistance in the form of polymorphisms in the guide RNA’s target DNA sequence.  Champer et al. (2017) have recently reported on their exploration of how Cas9 expression patterns as well as […]