melanogaster

Engineered Translocation-based Underdominance Gene Drive

A recent paper by Buchman et al. in ACS Synthetic Biology details the development of an underdominance effect through engineered chromosomal translocations in Drosophila melanogaster. The principle of using underdominance as a mechanism for driving desirable genes into populations has […]

Advances in CRISPR/Cas9 Genome Editing in Drosophila Lead the Way for Applications in Other Insects.

In the January edition of FlyBook (Genetics) Bier et al. review the recent advances in CRISPR/Cas technologies applied to the fruit fly, delving into the details of established and cutting-edge methods and their potential applications to future studies in this […]

Cas9 Expression Patterns Impact on Resistance Evolution in Different Genetic Backgrounds

Gene drive systems are sensitive to the evolution of resistance in the form of polymorphisms in the guide RNA’s target DNA sequence.  Champer et al. (2017) have recently reported on their exploration of how Cas9 expression patterns as well as […]

Genetic Manipulation of Insect Cell Lines

Two papers in the December issue of Genetics, Cherbas et al. 2015 and Manivannan et al. 2015, describe an improved method for making Drosophila cell lines with single, targeted mutations. Both works build upon existing recombination-mediated cassette exchange (RMCE) technology […]

Cas9-Mediated Gene-Drive In Mosquitoes

Gantz and Bier who demonstrated the fundamental principles of a Cas9-based gene drive system using Drosophila melanogaster in a publication earlier this year have recreated that system in the mosquito Anopheles stephensi with the help of collaborators.  Gantz et al. […]