knock-in

Recreating tra-2 Alleles in D. suzukii

Li and Handler (2017) have recently reported using CRISPR/Cas9 to make a temperature-sensitive transformer-2 (tra-2) allele in the pest species Drosophila suzukii by mutating the nucleotides orthologous to the Drosophila melanogaster tra-2ts2 allele. This study demonstrates an important proof of […]

High Knock in/out Rates in Aedes aegypti Expressing Cas9 from a Transgene.

  Li et al have published some interesting data on the preprint server bioRΧiv regarding the creation and use of Aedes aegypti lines expressing an SpCas9 transgene in the germline.  The report substantial improvements in gRNA-mediated mutagenesis rates. Cas9 mediated […]

Enhancing Homology Repair-Mediated Genome Editing

In a recent paper in Nature Biotechnology, Richardson et al (2016) report on the interaction of target-site bound Cas9 and dCas9 on DNA strand accessibility- and have identified an optimized approach to increase homologous recombination (HR) mediated repair. They found […]

Plasmids for Rapid and Versatile Genome Editing

As is the case for many other organisms, CRISPR has rapidly become the method of choice for targeted genome modification in insects. Much of the method development has been taking place in the model organism Drosophila melanogaster, where, in little […]