Watanabe et al in eNeuro (2018) report their successful development of transgenic cricket Gryllus bimaculatus that bears a transcriptional reporter for whole-brain, single cell-resolution activity mapping. In order to construct a transcriptional reporter for activity mapping, it is critical to […]
Gryllus
Dopamine receptor knock-outs in crickets
Awata et al. (2015) have used Cas9 to knock-out Dop1 in Gryllus bimaculatus to assess the role of dopamine neurons in mediating reward and punishment. Functional genomics technologies like transposons and first and second generation gene-editing technologies have been available […]
A Collection of Methods for Editing Insect Genomes
The August 2014 issue of METHODS is devoted to the topic of “Editing and Investigating Genomes with TALE and CRISPER/Cas Systems: Genome Engineering Across Species Using TALENS” Five of the 14 protocols included in this issue are specifically focused on […]
How to Edit a Cricket Genome: A Method
Crickets, and Gryllus bimaculatus in particular, are the subjects of research of neurobiologists, developmental biologist and others. Genetic technologies to foster the functional genomic analysis of these insects are in high demand even though these insects have a relatively long […]