gene silencing

Parental RNAi Via Oral Delivery

Khajuria et al (2015) have published a short paper in Insect Biochemistry and Molecular Biology describing their observations supporting the idea that Diabrotica vigifera exhibits parental RNAi following the oral delivery of dsRNA. Parental RNAi (pRNAi) refers to an inter-generational […]

RNAi-based therapeutics: Delivery is key and relevant to insects

Specific gene knockdown after exogenous small interfering RNA (siRNA) injection into humans and mammals holds great promise for therapeutic applications. But enthusiasm  waned and some predict its demise because of the manifold problems in delivering the technology. A new review […]

Tripodal RNA: An Enhanced RNAi Trigger

RNA interference-based manipulation of gene expression is a highly versatile tool available to insect scientists.  In some cases direct injection of large amounts of long dsRNA will suffice.  In other cases, not so much.  Combining siRNA with some kind of […]

Morpholino use in Mosquitoes is Promising

Antisense technologies have had limited application because oligodexoynucleotides in vivo have very limited stability. This stability problem due to rapid degradation of short DNA molecules limits the bioavailability of the molecules. Over the years there has been an evolution of […]