gene drive

A Synthetic Biology Approach Confers Resistance to Zika in Ae. aegypti

In a recent PNAS publication by Buchman et al (2019), the authors report on the establishment and validation of transgenic Aedes aegypti containing a synthetic anti-ZIKV cassette, capable of conferring complete refractoriness to the virus. Aided by piggyBac transposon-mediated insertion […]

Multiplexing gRNAs to Hedge Against Resistance to Gene Drive

Recently, Oberhofer et al (2018) published a paper examining the mechanisms of homing endonuclease gene drives in The Proceedings of the National Academy of Sciences. They used a novel nuclease-encoding cassette containing four multiplexed gRNAs targeting genes required for viability […]

Cas9 Gene Drive, Sex-Conversion and Evolved Resistance

In a recent manuscript, KaramiNejadRanjbar et al. demonstrate the development of a proof of principle Cas9-based suppression gene drive in D. melanogaster that can be applied to pest insects, and discuss the implications of resistance allele formation for practical use […]

Engineered Translocation-based Underdominance Gene Drive

A recent paper by Buchman et al. in ACS Synthetic Biology details the development of an underdominance effect through engineered chromosomal translocations in Drosophila melanogaster. The principle of using underdominance as a mechanism for driving desirable genes into populations has […]