Chemical and Light Inducible Recombinases

In a recent Nature Communications publication Weinberg et al., (2019) report developing and testing a repertoire of inducible site-specific recombinases capable of being activated by different inducers such as small molecules, light, and temperature.   While developed and tested in mammalian cells and transgenic mice, they will be widely useful in insect systems.

One-step recombination-mediated cassette exchange (RMCE) in Drosophila suzukii

Schetelig et al. (2018) describe a relatively efficient recombinase-mediated cassette exchange (RMCE) system for engineering the recently sequenced genome (Chiu et al., 2013) of the spotted wing drosophila, Drosophila suzukii, which is a significant pest of small and stone fruits. […]

Mosaic Analysis

The Recent review by Germani et al., on “Mosaic Analysis in Drosophila”, which appeared in January edition of Flybook of Genetics (http://www.genetics.org/content/208/2/473) discusses mosaicism, one of the finest and sophisticated techniques, which is not only well-utilized and established in Drosophila model […]

A Stable, Replaceable, Highly Efficient Transgene Expression System

Long et. al. in Scientific Reports describe a system for creating stable sites in the genome of Bombyx mori that allow for site-specific recombination-mediated integration of transgenes. Transposons are essential platforms for many insect genetic technologies and are typically used […]

Medusa: Harnessing a Sex-Linked Transgenic Drive System to Control Insect Populations

Segregation distorters (also known as drive genes) are selfish genetic elements with a natural fitness advantage – they are passed to greater than fifty percent of offspring in the next generation, breaking Mendel’s first law of inheritance. In nature, drive […]