editing

Gene Editing Spodoptera

Three papers recently described successful CRISPR/Cas9 mutagenesis in two species of Spodoptera.  Spodoptera litura and S. littoralis. Bi et al (2016) use Cas9 to create somatic mutations in S. litura in developmental gene abdominal-A, abd-A, and analyzed the resulting larvae […]

CHOPCHOP v2: Improved online CRISPR/Cas9 target prediction tool

In a paper published in Nucleic Acids Research, Labun et al. (2016), the developers of CHOPCHOP, report on their updated version: CHOPCHOPv2 . The first version of CHOPCHOP was released by a team of researchers from Harvard University a little […]

Phosphorothioate oligomers increase knock-in frequency and precision in TALEN- and Cas9-mediated genome editing

In a recent paper by Renaud et al. (2016), the authors investigate methods to increase genome editing knock-in efficiency by modifying the chemistry of the donor oligomers to be resistant to nuclease activity. The modifications they tested included locked nucleic […]

Dopamine receptor knock-outs in crickets

Awata et al. (2015) have used Cas9 to knock-out Dop1 in Gryllus bimaculatus to assess the role of dopamine neurons in mediating reward and punishment. Functional genomics technologies like transposons and first and second generation gene-editing technologies have been available […]

CRISPR/Cas9 Gene Editing in Papilionid Butterflies

Li et al (2015) recently published an exciting paper on butterflies genomics and functional genomics . Li et al have worked towards producing high quality genomes for extremely heterozygous and closely related butterflies P. xuthus and P. machaon. Further, they […]