dsRNA

A Protocol for the Highly Efficient Generation of Stable Mosquito Knockout Lines Using CRISPR/Cas9

In their recent paper in G3: Genes|Genomes|Genetics, Li et al. describe a highly efficient protocol for the generation of stable knockout lines using CRISPR/Cas9. The ability of effectively targeting and disrupting gene function is essential for gene functional analysis studies […]

Cas9 Mutagenesis in Houseflies Confirms Male Determining Factor Identification

Sharma et al. (2107) report in a recent issue of Science the identification of a male determining factor in the housefly Musca domestica and provide convincing reverse genetic confirmatory evidence using dsRNA-mediated gene silencing and Cas9-mediated mutagenesis. Alternative splicing of […]

Genetic Dissection of RNAi in the Colorado Potato Beetle, Leptinotarsa decemlineata

RNAi works well in beetles, in general, and why that is is not all that clear but Yoon et al. (2016) have begun to identify key genetic contributors in their recent paper in Insect Biochemistry and Molecular Biology.  The authors […]

Post-feeding and -injection Dynamics of dsRNA in 4 Insects

The variability of dsRNA-induced RNA interference is well documented with some insects very amenable to this method of regulating gene expression while are others are very much less so. The factors involved in this variation are known to some extent […]

Lipoplexes and oral dsRNA delivery to Blattella germanica

Lin et al., in Pesticide Management Science have described their efforts to orally deliver dsRNA to the German cockroach, Blattella germanica. Blattella germanica is fairly sensitive to RNAi when dsRNA is injected into the hemocoel.  Directly feeding B. germanica dsRNA […]

Carbon Quantum Dots for dsRNA Delivery

Das et al. (2015) in Applied Materials & Interfaces compare chitosan, silica and carbon quantum dot nanoparticles as dsRNA delivery platforms for silencing genes in larval mosquitoes. Direct injection of dsRNA into the hemocoel of insects has  been somewhat effective […]

Plastid Mediated RNAi: A Novel Insecticidal Tool

Recently, Zhang et al demonstrated a robust and promising way of controlling the devastating solanaceous crops insect pest, Colorado potato beetle (Leptinotarsa decemlineata; CPB), using a modified strategy for delivering plant mediated RNAi. Increasing insect herbivores’ resistance to plant defense mechanisms […]

Feed or Inject dsRNA in Aphids – It Depends

RNAi-based gene silencing has been reported but it has been problematic. The authors’ direct comparison of injection and feeding of dsRNA targeting the cathepsin-L gene in the pea aphid, Acyrthosiphon pisum. Single aphids were analyzed at the level of five […]