Drosophila

Multiplexing gRNAs to Hedge Against Resistance to Gene Drive

Recently, Oberhofer et al (2018) published a paper examining the mechanisms of homing endonuclease gene drives in The Proceedings of the National Academy of Sciences. They used a novel nuclease-encoding cassette containing four multiplexed gRNAs targeting genes required for viability […]

HyperActive piggyBac Transposase for Transgenesis

Eckermann et al. (2018) recently published a report in Insect Biochemistry and Molecular Biology demonstrating the efficacy of hyperactive piggyBac transposase for creating transgenic insects, which may be of interest to researchers looking to initiate transgenic projects in ‘non-model’ insect […]

Parallel Cloning by Site Specific Recombination

Blanco-Redondo and Langenhan (2018) report recently in G3 valuable improvements in the  φC31 system that enable the simultaneous targeted integration of two transgenes into distinct landing sites using a single source of  φC31 recombinase. The development of modern genetic engineering […]

Engineered Translocation-based Underdominance Gene Drive

A recent paper by Buchman et al. in ACS Synthetic Biology details the development of an underdominance effect through engineered chromosomal translocations in Drosophila melanogaster. The principle of using underdominance as a mechanism for driving desirable genes into populations has […]

Mosaic Analysis

The Recent review by Germani et al., on “Mosaic Analysis in Drosophila”, which appeared in January edition of Flybook of Genetics (http://www.genetics.org/content/208/2/473) discusses mosaicism, one of the finest and sophisticated techniques, which is not only well-utilized and established in Drosophila model […]

Recreating tra-2 Alleles in D. suzukii

Li and Handler (2017) have recently reported using CRISPR/Cas9 to make a temperature-sensitive transformer-2 (tra-2) allele in the pest species Drosophila suzukii by mutating the nucleotides orthologous to the Drosophila melanogaster tra-2ts2 allele. This study demonstrates an important proof of […]

Advances in CRISPR/Cas9 Genome Editing in Drosophila Lead the Way for Applications in Other Insects.

In the January edition of FlyBook (Genetics) Bier et al. review the recent advances in CRISPR/Cas technologies applied to the fruit fly, delving into the details of established and cutting-edge methods and their potential applications to future studies in this […]