Drosophila melanogaster

Cas9 Gene Drive, Sex-Conversion and Evolved Resistance

In a recent manuscript, KaramiNejadRanjbar et al. demonstrate the development of a proof of principle Cas9-based suppression gene drive in D. melanogaster that can be applied to pest insects, and discuss the implications of resistance allele formation for practical use […]

Systemic RNAi in Western Corn Rootworm, Diabrotica virgifera virgifera

Li et al. recently reported how systemic RNAi occurred in the western corn rootworm (WCR), Diabrotica virgifera virgifera, one of the most important maize pests in North America and a model for understanding the mechanism of RNAi. Efficiency of RNA […]

Transgenic Effectors for Neurogenic Manipulation

Genetic technologies will continue to play an increasingly important role in enabling the function of specific neurons in insect behavior to be determined. The genetic technologies available for manipulating Drosophila melanogaster provides not only great examples but some of these […]

Plasmids for Rapid and Versatile Genome Editing

As is the case for many other organisms, CRISPR has rapidly become the method of choice for targeted genome modification in insects. Much of the method development has been taking place in the model organism Drosophila melanogaster, where, in little […]

Medusa: Harnessing a Sex-Linked Transgenic Drive System to Control Insect Populations

Segregation distorters (also known as drive genes) are selfish genetic elements with a natural fitness advantage – they are passed to greater than fifty percent of offspring in the next generation, breaking Mendel’s first law of inheritance. In nature, drive […]

Transgenic Underdominance: Pushing Transgenes into Populations.

Insect population replacement and suppression using transgenic strategies are being considered for insects of medical and agricultural importance. Rapidly increasing the frequency of transgenes in target populations after transgene release using genetic drive systems remains a significant challenge. Driving transgenes […]