CRISPR

Gene Editing Screwworms and Blowflies

Paolo et al. report the first successful use of CRISPR/Cas9 in the New World Screwworm fly, Cochliomyia hominivorax (C. hominivorax), a parasite of warm blooded animals including humans, as well as a CRISPR/Cas9 protocol for the Australian sheep blowfly, Lucilia […]

CRISPR/Cas9-Mediated NHEJ Mutations in Anastrepha suspensa Paves Way for HDR

In an a recent experiment published in Gene, Li and Handler (2019) utilized readily available study systems and newly emerging genetic technologies and demonstrated both germline and somatic CRISPR/Cas9 induced mutations in Anastrepha ludens. The creation of heritable mutations was […]

Heritable Gene Editing in Thermobia domestica

Ohde et al report the first successful development and use of CRISPR/Cas9 in the basally branching insect Thermobia domestica. By co-injecting Cas9 nuclease with sgRNA targeting a T. domestica ortholog of the Drosophila melanogaster white gene (Td-w), the authors created […]

Microinjection of the Sand Fly Lutzomyia longipalpis, a Vector for Leishmaniasis

Two recent papers, Jefferies et al (2018) and Martin-Martin et. al (2018), describe experiments to develop and optimise a sand fly microinjection protocol. There are 2 authors to this post: Alla Madich, Simon Collier, technical constraints on this website allow […]

Cas9 Orthologs with Minimal PAM Requirements

In their recent paper Chatterjee and colleagues (2018) describe a natural protospacer adjacent motif (PAM) plasticity of previously uncharacterized Cas9 endonuclease from Streptococcus canis (ScCas9).  Chatterjee et al (2018) report ScCas9’s affinity to minimal 5’-NNG-3’ PAM sequences and describe its accurate editing capabilities, […]

CRISPR to go – a guide to gene editing Tephritid flies at remote facilities

Sim et al. (2018) recently published a paper in Insect Molecular Biology describing CRISPR/Cas 9 methods for gene editing in three genera of Tephritid fruit flies: Anastrepha, Bactrocera, and Ceratitis. CRISPR/Cas9 allows for targeted gene editing through homology driven repair […]