In a recent Nature Communications publication Weinberg et al., (2019) report developing and testing a repertoire of inducible site-specific recombinases capable of being activated by different inducers such as small molecules, light, and temperature. While developed and tested in mammalian cells and transgenic mice, they will be widely useful in insect systems.
Schetelig et al. (2018) describe a relatively efficient recombinase-mediated cassette exchange (RMCE) system for engineering the recently sequenced genome (Chiu et al., 2013) of the spotted wing drosophila, Drosophila suzukii, which is a significant pest of small and stone fruits. […]
Recently, Stroble et al. (2018) published in the journal, eLIFE, a novel technique to quickly and efficiently develop homozygous transgenic lines. Although this method was tested in the genetic model organism, Tribolium castaneum, it could be of particular interest for […]
Häcker et al 2017 reports the use of Cre/lox Recombination-Mediated Cassette Exchange (RMCE) in Aedes aegypti providing a pathway for interrogating specific genomic locations for the use of transgenic constructs in future genetic control methods. Häcker et al 2017 first generated […]
Woodard and Wilson (2015) have recently published an admirable review in Trends in Biotechnology of the diverse applications of the piggyBac transposon system in mammalian cells. Insect biologists working with or interested in piggyBac will be interested in reading this […]
Volohonsky et. al. (2015) in a just-published paper in G3 entitled “Tools for Anopheles gambiae transgenesis” describe a wealth of new tools for creating transgenic An. gambiae. There has been a protocol for Anopheles gambiae transgenesis for over 15 years, […]
Long et. al. in Scientific Reports describe a system for creating stable sites in the genome of Bombyx mori that allow for site-specific recombination-mediated integration of transgenes. Transposons are essential platforms for many insect genetic technologies and are typically used […]