Intramolecular interactions in gRNA may influence Cas9 cleavage activity

In an article recently published in Nature Communications, Thyme et al (2016) discuss the interactions of the sequences of sgRNAs, the role of chromatin, and how these factors might interfere with Cas9 cleavage activity. By investigating the activity of Cas9 […]

CHOPCHOP v2: Improved online CRISPR/Cas9 target prediction tool

In a paper published in Nucleic Acids Research, Labun et al. (2016), the developers of CHOPCHOP, report on their updated version: CHOPCHOPv2 . The first version of CHOPCHOP was released by a team of researchers from Harvard University a little […]