Cas9

ReMOT control delivery technology holds promise for CRISPR-Cas9 activity independent of embryonic injection

A new report in Nature Communications by Chaverra-Rodriguez et al. details a novel Cas9-delivery approach for insect genetic modification. In their work, Chaverra-Rodriguez et al introduce a technology they term “ReMOT control”, or Receptor-Mediated Ovary Transduction of Cargo. In their […]

Target Site Accessibility and Development of Better Guide RNA Design Tools For CRISPR/Cas9

A recent paper by the Lee et al (2018) has shown that the present CRISPR/Cas9 design tools do not accurately predict the activity of single-gene targeting gRNA sequences. They also report that the non sequence based features like DNA accessibility […]

Efficient CRISPR/Cas9 Knockout of vermillion in Helicoverpa zea (Boddie)

  Perera et al. (2018) recently used CRISPR/Cas gene editing in Helicoverpa zea, a lepidopteran pest of many crops. The authors showed positive correlations between different concentrations of ribonucleoprotein (RNP) complexes and Cas9 protein with mutation percentage of the vermillion […]

Precise and Efficient Nucleotide Substitution Via Noncanonical Homology-directed Repair.

In a recent paper in Genome Research Nakajima et al. 2018 propose an efficient gene editing strategy by nucleotide substitution that overcomes three general problems associated with current methods. First, indels caused by the traditional Cas9-induced Double Strand Breaks (DSB), […]