Bombyx

Two Bombyx Testis-Specific Promoters

The level of sophistication with which one can genetically manipulate insects is very much determined by how well transgene expression can be regulated. Xu et al (2014) in Insect Molecular Biology describe their successful efforts to isolate promoters from two […]

Transgenic Effectors for Neurogenic Manipulation

Genetic technologies will continue to play an increasingly important role in enabling the function of specific neurons in insect behavior to be determined. The genetic technologies available for manipulating Drosophila melanogaster provides not only great examples but some of these […]

A Collection of Methods for Editing Insect Genomes

The August 2014 issue of METHODS is devoted to the topic of “Editing and Investigating Genomes with TALE and CRISPER/Cas Systems: Genome Engineering Across Species Using TALENS” Five of the 14 protocols included in this issue are specifically focused on […]

Transgenic TALEN Expression to Control Sex in Bombyx

Analysis of gene function in insects by editing the genome, or reverse genetics, is now greatly facilitated thanks to powerful genetic technologies, such as TALENs, ZFNs, and more recently CRISPR/Cas9, becoming available in many organisms. However, screening of transgenic mutated […]

Strongly and Ubiquitously Expressed Promoter for Lepidoptera

The number of genetic technologies available for the study of the silkworm, Bombyx mori, is second only to those available for Drosophila melanogaster. Germ-line transformation, enhancer-trapping, genome editing/modification using CRISPRs, TALENS, ZFNs  are all established technologies in B. mori. But, […]