Anopheles

Cas9-Mediated Gene-Drive In Mosquitoes

Gantz and Bier who demonstrated the fundamental principles of a Cas9-based gene drive system using Drosophila melanogaster in a publication earlier this year have recreated that system in the mosquito Anopheles stephensi with the help of collaborators.  Gantz et al. […]

Anopheles gambiae Transgenesis Tool Box – Major Additions

Volohonsky et. al. (2015) in a just-published paper in G3 entitled  “Tools for Anopheles gambiae transgenesis”  describe a wealth of new tools for creating transgenic An. gambiae. There has been a protocol for Anopheles gambiae transgenesis for over 15 years, […]

New ΦC31 Resources

A rapid method for generating and finding ΦC31 landing sites (attP) with the desired expression characteristics as well as a new method for creating transgene arrays in vivo was recently described by Knapp et al (2015). Albeit these methods were […]

Towards a Medea Drive System in Anopheles Mosquitoes

Germ-line specific promoters are useful to insect biologists and Biedler and colleagues (2015) in a just-published paper in G3-Genes|Genomes|Genetics report their efforts to identify, isolate and test maternal germline-specific genes and promoters in the Asian malaria vector, Anopheles stephensi. “We […]

Transgenic Effectors for Neurogenic Manipulation

Genetic technologies will continue to play an increasingly important role in enabling the function of specific neurons in insect behavior to be determined. The genetic technologies available for manipulating Drosophila melanogaster provides not only great examples but some of these […]

Medusa: Harnessing a Sex-Linked Transgenic Drive System to Control Insect Populations

Segregation distorters (also known as drive genes) are selfish genetic elements with a natural fitness advantage – they are passed to greater than fifty percent of offspring in the next generation, breaking Mendel’s first law of inheritance. In nature, drive […]

Morpholino use in Mosquitoes is Promising

Antisense technologies have had limited application because oligodexoynucleotides in vivo have very limited stability. This stability problem due to rapid degradation of short DNA molecules limits the bioavailability of the molecules. Over the years there has been an evolution of […]

Advanced Mosquito Genetic Engineering

For mosquitoes the number of genetic modification technologies that can and have been applied continues to grow, and while still not as deep as the Drosophila toolbox the mosquito toolbox is still impressive in the right hands. Bernardini et al. […]