Limitations of Per Os Delivery of dsRNA to Schistocerca

Schistocera gregaria

Schistocera gregaria

RNA interference (RNAi) has become a widely used insect genetic technology and holds great potential to contribute to the development of novel strategies for insect pest control.

There have been some notable reports of per os delivery of RNAi even when expressed in plant tissue.

While previous studies clearly demonstrated that injection of dsRNA into the body cavity of the desert locust, Schistocerca gregaria, is highly effective to induce gene silencing effects, Wynant et al. observed that the RNAi response is much less sensitive to orally delivered dsRNA.

The presence of dsRNA degrading enzymes in the gut limit per os delivery in this species.


Wynant, Niels; Santos, Dulce; Verdonck, Rik; et al. Identification, functional characterization and phylogenetic analysis of double stranded RNA degrading enzymes present in the gut of the desert locust, Schistocerca gregaria, Insect Biochemistry And Molecular Biology , Volume: 46, Pages: 1-8 , DOI:  10.1016/j.ibmb.2013.12.008




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