Technology Topics

This is a community blog to capture ongoing developments in and applications of insect genetic technologies.
IGTRCN participants are encouraged to post to this blog when they find a Technology Topic of interest.
Use the Submissions button at the bottom of this page and select Technology Topics or comment directly on existing posts.
The search function or the Tag Field at the bottom of this page can help you locate topics of interest.
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Heritable Gene Editing in Thermobia domestica

Ohde et al report the first successful development and use of CRISPR/Cas9 in the basally branching insect Thermobia domestica. By co-injecting Cas9 nuclease with sgRNA targeting a T. domestica ortholog of the Drosophila melanogaster white gene (Td-w), the authors created […]

Improved Honeybee Germline Transformation

Otte et al (2018) build upon their previous work (Schulte et al. 2014) in this new publication and demonstrate a twofold improved efficiency at generating transgenic honeybees using the piggyBac transposon system.  The inability to generate high transformation rates has […]

2019 IGTRCN Technical Short Course – Applications Open

2019 IGTRCN Technical Short Course on Insect Genetic Technologies: Theory and Practice July 21 – 26, 2019 The Insect Transformation Facility Institute for Bioscience and Biotechnology Research University of Maryland College Park Applications by April 1, 2019 (24 seats available) […]

Enhancer Trapping in Mosquitoes

A recent manuscript published on Genes Genomes Genetics by Reid et al (2018) reports an exciting improvement to piggyBac-based promoter-trap designed in Anopheles stephensi. The development of this new piggyBac-based promoter-trap designs provides further knowledge and exciting venues to examine […]

Cas9 Orthologs with Minimal PAM Requirements

In their recent paper Chatterjee and colleagues (2018) describe a natural protospacer adjacent motif (PAM) plasticity of previously uncharacterized Cas9 endonuclease from Streptococcus canis (ScCas9).  Chatterjee et al (2018) report ScCas9’s affinity to minimal 5’-NNG-3’ PAM sequences and describe its accurate editing capabilities, […]

Precision-Guided Sterile Insect Technique

Right after the 2019 New Year, Kandul et al (2019) described a novel CRISPR-based technology which they termed as “precision guided sterile insect technique (pgSIT)”. The idea of traditional SIT was planted in 1930s, which uses radiation to induce sterility […]

A Multiple Gene Drive System

Yan & Finnigan, (2018) recently published a paper in Scientific Reports describing an artificial multi-locus gene drive system by using a single Cas9 and three guide RNA (gRNA) in the budding yeast Saccharomyces cerevisiae. Nuclease-based gene drives do not follow […]

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