Kyrou et al. (2018) in a recent paper in Nature Biotechnology report identifying a highly conserved target site in the Anopheles gambiae fertility gene doublesex (dsx), that cannot tolerate mutations. They built a gene drive targeting this site in dsx […]
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Zhang et al (2018) in a recent paper in PNAS report their successful integration of transgenes into the W chromosome of Bombyx mori. Initially, they set out to place visible markers on the W. This would enable rapid and accurate […]
Jose Arturo Gutierrez-Triana and colleagues from Heidelberg University report in eLIFE a CRISPR/Cas9 based methodology that simultaneously decreases donor DNA multimerization and increases HDR single-copy integration. A common obstacle that CRISPR/Cas HDR mediated genome editing faces is the accurate and […]
Nishimasu et al. (2018) recently reported in Science on their finds related to a new SpCas9 modification referred as SpCas9-NG that has an expanded target range. The authors directionally mutated the wild-type SpCas9 enzyme to achieve its relaxed preferences to the […]
A recent paper by Li et al (2018) published in The Proceedings of the National Academy of Sciences, demonstrated an effective method for enhancing the RNA interference (RNAi) by codelivery of siRNA and Argonaute 2 (Ago-2) protein complex through different […]
Blanco-Redondo and Langenhan (2018) report recently in G3 valuable improvements in the φC31 system that enable the simultaneous targeted integration of two transgenes into distinct landing sites using a single source of φC31 recombinase. The development of modern genetic engineering […]
In a recent manuscript, KaramiNejadRanjbar et al. demonstrate the development of a proof of principle Cas9-based suppression gene drive in D. melanogaster that can be applied to pest insects, and discuss the implications of resistance allele formation for practical use […]
Buchman et al (2018) from Tata Institute of California University reported in Insect Molecular Biology the use of CRISPR/Cas9-HDR system in order to insert transgenes to specific sites of the Drosophila melanogaster Y chromosome. In addition they investigated its usage […]
A recent manuscript by Suzuki et al (2018) demonstrates an effective method to control the expression of CRISPR Cas9 based molecular components with a small molecule. Existing methods for controlling Cas9 suffer from high background activity and/or use compounds […]
A recent paper in Frontiers in Physiology by Taning et al. demonstrated the feasibility of engineering an insect virus into a delivery system capable of eliciting a gene silencing response, through the RNAi pathway, both in vitro and in vivo […]