Technology Topics

This is a community blog to capture ongoing developments in and applications of insect genetic technologies.
IGTRCN participants are encouraged to post to this blog when they find a Technology Topic of interest.
Use the Submissions button at the bottom of this page and select Technology Topics or comment directly on existing posts.
The search function or the Tag Field at the bottom of this page can help you locate topics of interest.
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Driving into doublesex

Kyrou et al. (2018) in a recent paper in Nature Biotechnology report identifying a highly conserved target site in the Anopheles gambiae fertility gene doublesex (dsx), that cannot tolerate mutations. They built a gene drive targeting this site in dsx […]

Improved HDR-Mediated Genome Editing

Jose Arturo Gutierrez-Triana and colleagues from Heidelberg University report in eLIFE a CRISPR/Cas9 based methodology that simultaneously decreases donor DNA multimerization and increases HDR single-copy integration. A common obstacle that CRISPR/Cas HDR mediated genome editing faces is the accurate and […]

New SpCas9 With Expanded Target Range

Nishimasu et al. (2018) recently reported in Science on their finds related to a new SpCas9 modification referred as SpCas9-NG that has an expanded target range. The authors directionally mutated the wild-type SpCas9 enzyme to achieve its relaxed preferences to the […]

Parallel Cloning by Site Specific Recombination

Blanco-Redondo and Langenhan (2018) report recently in G3 valuable improvements in the  φC31 system that enable the simultaneous targeted integration of two transgenes into distinct landing sites using a single source of  φC31 recombinase. The development of modern genetic engineering […]

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