A recent manuscript posted on bioRiv by Hammond et al (2018) report exciting improvements to CRISPR-based suppression gene drives in Anopheles gambiae, with the development of new CRISPR designs that reduce selection of resistance and results in much higher suppression […]
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ReMOT control delivery technology holds promise for CRISPR-Cas9 activity independent of embryonic injection
A new report in Nature Communications by Chaverra-Rodriguez et al. details a novel Cas9-delivery approach for insect genetic modification. In their work, Chaverra-Rodriguez et al introduce a technology they term “ReMOT control”, or Receptor-Mediated Ovary Transduction of Cargo. In their […]
A recent paper by the Lee et al (2018) has shown that the present CRISPR/Cas9 design tools do not accurately predict the activity of single-gene targeting gRNA sequences. They also report that the non sequence based features like DNA accessibility […]
INVITATION FOR 1ST INTERNATIONAL MOLECULAR PLANT PROTECTION CONGRESS-ADANA, TURKEY, APRIL 10-13, 2019 http://www.imppc2019.org/ Dear Colleagues, Molecular approaches are currently among the most popular topics in biological sciences and provide many opportunities in the solution of various problems. We have witnessed […]
A recent paper by Buchman et al. in ACS Synthetic Biology details the development of an underdominance effect through engineered chromosomal translocations in Drosophila melanogaster. The principle of using underdominance as a mechanism for driving desirable genes into populations has […]
A recent modelling study by Dhole et al. in Evolutionary Applications has thus sought to compare the invasiveness of three gene drives previously described as either spatially or temporally self-limiting. Recent discussions on gene drives have begun to shift away […]
Perera et al. (2018) recently used CRISPR/Cas gene editing in Helicoverpa zea, a lepidopteran pest of many crops. The authors showed positive correlations between different concentrations of ribonucleoprotein (RNP) complexes and Cas9 protein with mutation percentage of the vermillion […]
In a recent PNAS article, Champer et al. report variable rates of gene drive-based resistance allele formation. Gene drives have shown early promise as the next big thing in controlling insects both for the benefit of agricultural production and reducing […]
In a recent paper in Genome Research Nakajima et al. 2018 propose an efficient gene editing strategy by nucleotide substitution that overcomes three general problems associated with current methods. First, indels caused by the traditional Cas9-induced Double Strand Breaks (DSB), […]
Komor et al. reviewed two strategies for genome editing that do not rely on the Double Strand Breaks (DSB) in a recent issue ACS Chemical Biology. The authors specifically address oligonucleotide-directed mutagenesis and base pairing. Possibly the most harmful type […]