In their recent paper Chatterjee and colleagues (2018) describe a natural protospacer adjacent motif (PAM) plasticity of previously uncharacterized Cas9 endonuclease from Streptococcus canis (ScCas9). Chatterjee et al (2018) report ScCas9’s affinity to minimal 5’-NNG-3’ PAM sequences and describe its accurate editing capabilities, […]
This is a community blog to capture ongoing developments in and applications of insect genetic technologies.
IGTRCN participants are encouraged to post to this blog when they find a Technology Topic of interest.
Use the Submissions button at the bottom of this page and select Technology Topics or comment directly on existing posts.
The search function or the Tag Field at the bottom of this page can help you locate topics of interest.
There will be a short delay before your post appears.
Right after the 2019 New Year, Kandul et al (2019) described a novel CRISPR-based technology which they termed as “precision guided sterile insect technique (pgSIT)”. The idea of traditional SIT was planted in 1930s, which uses radiation to induce sterility […]
Yan & Finnigan, (2018) recently published a paper in Scientific Reports describing an artificial multi-locus gene drive system by using a single Cas9 and three guide RNA (gRNA) in the budding yeast Saccharomyces cerevisiae. Nuclease-based gene drives do not follow […]
Schetelig et al. (2018) describe a relatively efficient recombinase-mediated cassette exchange (RMCE) system for engineering the recently sequenced genome (Chiu et al., 2013) of the spotted wing drosophila, Drosophila suzukii, which is a significant pest of small and stone fruits. […]
2019 IGTRCN Technical Short Course on Insect Genetic Technologies: Theory and Practice July 21 – 26, 2019 The Insect Transformation Facility Institute for Bioscience and Biotechnology Research University of Maryland College Park Applications by April 1, 2019 (24 seats available) […]
Sim et al. (2018) recently published a paper in Insect Molecular Biology describing CRISPR/Cas 9 methods for gene editing in three genera of Tephritid fruit flies: Anastrepha, Bactrocera, and Ceratitis. CRISPR/Cas9 allows for targeted gene editing through homology driven repair […]
Watanabe et al in eNeuro (2018) report their successful development of transgenic cricket Gryllus bimaculatus that bears a transcriptional reporter for whole-brain, single cell-resolution activity mapping. In order to construct a transcriptional reporter for activity mapping, it is critical to […]
Recently, Oberhofer et al (2018) published a paper examining the mechanisms of homing endonuclease gene drives in The Proceedings of the National Academy of Sciences. They used a novel nuclease-encoding cassette containing four multiplexed gRNAs targeting genes required for viability […]
Eckermann et al. (2018) recently published a report in Insect Biochemistry and Molecular Biology demonstrating the efficacy of hyperactive piggyBac transposase for creating transgenic insects, which may be of interest to researchers looking to initiate transgenic projects in ‘non-model’ insect […]
Kyrou et al. (2018) in a recent paper in Nature Biotechnology report identifying a highly conserved target site in the Anopheles gambiae fertility gene doublesex (dsx), that cannot tolerate mutations. They built a gene drive targeting this site in dsx […]