Technology Topics

This is a community blog to capture ongoing developments in and applications of insect genetic technologies.
IGTRCN participants are encouraged to post to this blog when they find a Technology Topic of interest.
Use the Submissions button at the bottom of this page and select Technology Topics or comment directly on existing posts.
The search function or the Tag Field at the bottom of this page can help you locate topics of interest.
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A Multiple Gene Drive System

Yan & Finnigan, (2018) recently published a paper in Scientific Reports describing an artificial multi-locus gene drive system by using a single Cas9 and three guide RNA (gRNA) in the budding yeast Saccharomyces cerevisiae. Nuclease-based gene drives do not follow […]

Driving into doublesex

Kyrou et al. (2018) in a recent paper in Nature Biotechnology report identifying a highly conserved target site in the Anopheles gambiae fertility gene doublesex (dsx), that cannot tolerate mutations. They built a gene drive targeting this site in dsx […]

Improved HDR-Mediated Genome Editing

Jose Arturo Gutierrez-Triana and colleagues from Heidelberg University report in eLIFE a CRISPR/Cas9 based methodology that simultaneously decreases donor DNA multimerization and increases HDR single-copy integration. A common obstacle that CRISPR/Cas HDR mediated genome editing faces is the accurate and […]

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