Yan & Finnigan, (2018) recently published a paper in Scientific Reports describing an artificial multi-locus gene drive system by using a single Cas9 and three guide RNA (gRNA) in the budding yeast Saccharomyces cerevisiae. Nuclease-based gene drives do not follow […]
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Schetelig et al. (2018) describe a relatively efficient recombinase-mediated cassette exchange (RMCE) system for engineering the recently sequenced genome (Chiu et al., 2013) of the spotted wing drosophila, Drosophila suzukii, which is a significant pest of small and stone fruits. […]
2019 IGTRCN Technical Short Course on Insect Genetic Technologies: Theory and Practice July 21 – 26, 2019 The Insect Transformation Facility Institute for Bioscience and Biotechnology Research University of Maryland College Park Applications by April 1, 2019 (24 seats available) […]
Sim et al. (2018) recently published a paper in Insect Molecular Biology describing CRISPR/Cas 9 methods for gene editing in three genera of Tephritid fruit flies: Anastrepha, Bactrocera, and Ceratitis. CRISPR/Cas9 allows for targeted gene editing through homology driven repair […]
Watanabe et al in eNeuro (2018) report their successful development of transgenic cricket Gryllus bimaculatus that bears a transcriptional reporter for whole-brain, single cell-resolution activity mapping. In order to construct a transcriptional reporter for activity mapping, it is critical to […]
Recently, Oberhofer et al (2018) published a paper examining the mechanisms of homing endonuclease gene drives in The Proceedings of the National Academy of Sciences. They used a novel nuclease-encoding cassette containing four multiplexed gRNAs targeting genes required for viability […]
Eckermann et al. (2018) recently published a report in Insect Biochemistry and Molecular Biology demonstrating the efficacy of hyperactive piggyBac transposase for creating transgenic insects, which may be of interest to researchers looking to initiate transgenic projects in ‘non-model’ insect […]
Kyrou et al. (2018) in a recent paper in Nature Biotechnology report identifying a highly conserved target site in the Anopheles gambiae fertility gene doublesex (dsx), that cannot tolerate mutations. They built a gene drive targeting this site in dsx […]
Zhang et al (2018) in a recent paper in PNAS report their successful integration of transgenes into the W chromosome of Bombyx mori. Initially, they set out to place visible markers on the W. This would enable rapid and accurate […]
Jose Arturo Gutierrez-Triana and colleagues from Heidelberg University report in eLIFE a CRISPR/Cas9 based methodology that simultaneously decreases donor DNA multimerization and increases HDR single-copy integration. A common obstacle that CRISPR/Cas HDR mediated genome editing faces is the accurate and […]