A recent publication by Strecker et al., 2019, opens up the intriguing possibility that some transposons may be able to be guided to specific sites in the genome thereby overcoming perhaps the greatest hurdle that prevents them from being used […]
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Barkau et. al. 2019 report the development of small nucleic acid-based inhibitors (SNuBs) of SpCas9. Due to considerations such as off-target activity, it is desirable that we have a high degree of control over CRISPR/Cas9 cleavage rates. However, while several […]
In an a recent experiment published in Gene, Li and Handler (2019) utilized readily available study systems and newly emerging genetic technologies and demonstrated both germline and somatic CRISPR/Cas9 induced mutations in Anastrepha ludens. The creation of heritable mutations was […]
The “Arthropod Containment Guidelines” developed by The American Committee of Medical Entomology, a Committee within the American Society of Tropical Medicine and Hygiene have been revised and published in Vector-Borne and Zoonotic Diseaese. The ACGs were originally published in 2004 […]
Ohde et al report the first successful development and use of CRISPR/Cas9 in the basally branching insect Thermobia domestica. By co-injecting Cas9 nuclease with sgRNA targeting a T. domestica ortholog of the Drosophila melanogaster white gene (Td-w), the authors created […]
Otte et al (2018) build upon their previous work (Schulte et al. 2014) in this new publication and demonstrate a twofold improved efficiency at generating transgenic honeybees using the piggyBac transposon system. The inability to generate high transformation rates has […]
2019 IGTRCN Technical Short Course on Insect Genetic Technologies: Theory and Practice July 21 – 26, 2019 The Insect Transformation Facility Institute for Bioscience and Biotechnology Research University of Maryland College Park Applications by April 1, 2019 (24 seats available) […]
In a recent PNAS publication by Buchman et al (2019), the authors report on the establishment and validation of transgenic Aedes aegypti containing a synthetic anti-ZIKV cassette, capable of conferring complete refractoriness to the virus. Aided by piggyBac transposon-mediated insertion […]
A recent manuscript published on Genes Genomes Genetics by Reid et al (2018) reports an exciting improvement to piggyBac-based promoter-trap designed in Anopheles stephensi. The development of this new piggyBac-based promoter-trap designs provides further knowledge and exciting venues to examine […]
Two recent papers, Jefferies et al (2018) and Martin-Martin et. al (2018), describe experiments to develop and optimise a sand fly microinjection protocol. There are 2 authors to this post: Alla Madich, Simon Collier, technical constraints on this website allow […]