Buchman et al (2018) from Tata Institute of California University reported in Insect Molecular Biology the use of CRISPR/Cas9-HDR system in order to insert transgenes to specific sites of the Drosophila melanogaster Y chromosome. In addition they investigated its usage […]
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A recent manuscript by Suzuki et al (2018) demonstrates an effective method to control the expression of CRISPR Cas9 based molecular components with a small molecule. Existing methods for controlling Cas9 suffer from high background activity and/or use compounds […]
A recent paper in Frontiers in Physiology by Taning et al. demonstrated the feasibility of engineering an insect virus into a delivery system capable of eliciting a gene silencing response, through the RNAi pathway, both in vitro and in vivo […]
Nan Cher Yeo and colleagues in the Church lab report in Nature Methods the identification of a combination of repressor domains which increases gene repression and could be widely useful. The CRISPR-Cas system is widely used as a powerful gene […]
A recent manuscript posted on bioRiv by Hammond et al (2018) report exciting improvements to CRISPR-based suppression gene drives in Anopheles gambiae, with the development of new CRISPR designs that reduce selection of resistance and results in much higher suppression […]
ReMOT control delivery technology holds promise for CRISPR-Cas9 activity independent of embryonic injection
A new report in Nature Communications by Chaverra-Rodriguez et al. details a novel Cas9-delivery approach for insect genetic modification. In their work, Chaverra-Rodriguez et al introduce a technology they term “ReMOT control”, or Receptor-Mediated Ovary Transduction of Cargo. In their […]
A recent paper by the Lee et al (2018) has shown that the present CRISPR/Cas9 design tools do not accurately predict the activity of single-gene targeting gRNA sequences. They also report that the non sequence based features like DNA accessibility […]
INVITATION FOR 1ST INTERNATIONAL MOLECULAR PLANT PROTECTION CONGRESS-ADANA, TURKEY, APRIL 10-13, 2019 http://www.imppc2019.org/ Dear Colleagues, Molecular approaches are currently among the most popular topics in biological sciences and provide many opportunities in the solution of various problems. We have witnessed […]
A recent paper by Buchman et al. in ACS Synthetic Biology details the development of an underdominance effect through engineered chromosomal translocations in Drosophila melanogaster. The principle of using underdominance as a mechanism for driving desirable genes into populations has […]
A recent modelling study by Dhole et al. in Evolutionary Applications has thus sought to compare the invasiveness of three gene drives previously described as either spatially or temporally self-limiting. Recent discussions on gene drives have begun to shift away […]