Technology Topics

This is a community blog to capture ongoing developments in and applications of insect genetic technologies.
IGTRCN participants are encouraged to post to this blog when they find a Technology Topic of interest.
Use the Submissions button at the bottom of this page and select Technology Topics or comment directly on existing posts.
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Gene Editing Screwworms and Blowflies

Paolo et al. report the first successful use of CRISPR/Cas9 in the New World Screwworm fly, Cochliomyia hominivorax (C. hominivorax), a parasite of warm blooded animals including humans, as well as a CRISPR/Cas9 protocol for the Australian sheep blowfly, Lucilia […]

“Sexing” Technologies – A Review

In a recent review,  Lutrat et al. (2019) provide a comprehensive summary of current (since 2003) sexing methods available for various dipteran pest species. The review distinguishes between early and late acting sexing methods and elaborates on the techniques used […]

Homology-Free Knockins in Insects

Bosch et. al. 2019 report in biorxiv a homology-independent genomic site-specific gene knock-in method that can be applied both in vitro and in vivo. Traditional methods of site-specific knock-in use homologous repair, which requires cloning of long homology arms that […]

Small Nucleic Acid Based Inhibitors of SpCas9 (SNuBs)

Barkau et. al. 2019 report the development of small nucleic acid-based inhibitors (SNuBs) of SpCas9.  Due to considerations such as off-target activity, it is desirable that we have a high degree of control over CRISPR/Cas9 cleavage rates. However, while several […]

Revised Arthropod Containment Guideline

The  “Arthropod Containment Guidelines” developed by The American Committee of Medical Entomology, a Committee within the American Society of Tropical Medicine and Hygiene have been revised and published in Vector-Borne and Zoonotic Diseaese. The ACGs were originally published in 2004 […]

Heritable Gene Editing in Thermobia domestica

Ohde et al report the first successful development and use of CRISPR/Cas9 in the basally branching insect Thermobia domestica. By co-injecting Cas9 nuclease with sgRNA targeting a T. domestica ortholog of the Drosophila melanogaster white gene (Td-w), the authors created […]

Improved Honeybee Germline Transformation

Otte et al (2018) build upon their previous work (Schulte et al. 2014) in this new publication and demonstrate a twofold improved efficiency at generating transgenic honeybees using the piggyBac transposon system.  The inability to generate high transformation rates has […]

2019 IGTRCN Technical Short Course – Applications Open

2019 IGTRCN Technical Short Course on Insect Genetic Technologies: Theory and Practice July 21 – 26, 2019 The Insect Transformation Facility Institute for Bioscience and Biotechnology Research University of Maryland College Park Applications by April 1, 2019 (24 seats available) […]

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