Nishimasu et al. (2018) recently reported in Science on their finds related to a new SpCas9 modification referred as SpCas9-NG that has an expanded target range. The authors directionally mutated the wild-type SpCas9 enzyme to achieve its relaxed preferences to the […]
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A recent paper by Li et al (2018) published in The Proceedings of the National Academy of Sciences, demonstrated an effective method for enhancing the RNA interference (RNAi) by codelivery of siRNA and Argonaute 2 (Ago-2) protein complex through different […]
Blanco-Redondo and Langenhan (2018) report recently in G3 valuable improvements in the φC31 system that enable the simultaneous targeted integration of two transgenes into distinct landing sites using a single source of φC31 recombinase. The development of modern genetic engineering […]
In a recent manuscript, KaramiNejadRanjbar et al. demonstrate the development of a proof of principle Cas9-based suppression gene drive in D. melanogaster that can be applied to pest insects, and discuss the implications of resistance allele formation for practical use […]
Buchman et al (2018) from Tata Institute of California University reported in Insect Molecular Biology the use of CRISPR/Cas9-HDR system in order to insert transgenes to specific sites of the Drosophila melanogaster Y chromosome. In addition they investigated its usage […]
A recent manuscript by Suzuki et al (2018) demonstrates an effective method to control the expression of CRISPR Cas9 based molecular components with a small molecule. Existing methods for controlling Cas9 suffer from high background activity and/or use compounds […]
A recent paper in Frontiers in Physiology by Taning et al. demonstrated the feasibility of engineering an insect virus into a delivery system capable of eliciting a gene silencing response, through the RNAi pathway, both in vitro and in vivo […]
Nan Cher Yeo and colleagues in the Church lab report in Nature Methods the identification of a combination of repressor domains which increases gene repression and could be widely useful. The CRISPR-Cas system is widely used as a powerful gene […]
A recent manuscript posted on bioRiv by Hammond et al (2018) report exciting improvements to CRISPR-based suppression gene drives in Anopheles gambiae, with the development of new CRISPR designs that reduce selection of resistance and results in much higher suppression […]
ReMOT control delivery technology holds promise for CRISPR-Cas9 activity independent of embryonic injection
A new report in Nature Communications by Chaverra-Rodriguez et al. details a novel Cas9-delivery approach for insect genetic modification. In their work, Chaverra-Rodriguez et al introduce a technology they term “ReMOT control”, or Receptor-Mediated Ovary Transduction of Cargo. In their […]