Traditional genome editing in insects involves directly injecting embryos. However, most insect embryos are small and sensitive to manipulation.
In a recent Nature Communications publication Weinberg et al., (2019) report developing and testing a repertoire of inducible site-specific recombinases capable of being activated by different inducers such as small molecules, light, and temperature. While developed and tested in mammalian cells and transgenic mice, they will be widely useful in insect systems.
Paolo et al. report the first successful use of CRISPR/Cas9 in the New World Screwworm fly, Cochliomyia hominivorax (C. hominivorax), a parasite of warm blooded animals including humans, as well as a CRISPR/Cas9 protocol for the Australian sheep blowfly, Lucilia […]
In a recent review, Lutrat et al. (2019) provide a comprehensive summary of current (since 2003) sexing methods available for various dipteran pest species. The review distinguishes between early and late acting sexing methods and elaborates on the techniques used […]
Bosch et. al. 2019 report in biorxiv a homology-independent genomic site-specific gene knock-in method that can be applied both in vitro and in vivo. Traditional methods of site-specific knock-in use homologous repair, which requires cloning of long homology arms that […]
A recent publication by Strecker et al., 2019, opens up the intriguing possibility that some transposons may be able to be guided to specific sites in the genome thereby overcoming perhaps the greatest hurdle that prevents them from being used […]
Barkau et. al. 2019 report the development of small nucleic acid-based inhibitors (SNuBs) of SpCas9. Due to considerations such as off-target activity, it is desirable that we have a high degree of control over CRISPR/Cas9 cleavage rates. However, while several […]
In an a recent experiment published in Gene, Li and Handler (2019) utilized readily available study systems and newly emerging genetic technologies and demonstrated both germline and somatic CRISPR/Cas9 induced mutations in Anastrepha ludens. The creation of heritable mutations was […]