Technology Topics

This is a community blog to capture ongoing developments in and applications of insect genetic technologies.
IGTRCN participants are encouraged to post to this blog when they find a Technology Topic of interest.
Use the Submissions button at the bottom of this page and select Technology Topics or comment directly on existing posts.
The search function or the Tag Field at the bottom of this page can help you locate topics of interest.
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New SpCas9 With Expanded Target Range

Nishimasu et al. (2018) recently reported in Science on their finds related to a new SpCas9 modification referred as SpCas9-NG that has an expanded target range. The authors directionally mutated the wild-type SpCas9 enzyme to achieve its relaxed preferences to the […]

Parallel Cloning by Site Specific Recombination

Blanco-Redondo and Langenhan (2018) report recently in G3 valuable improvements in the  φC31 system that enable the simultaneous targeted integration of two transgenes into distinct landing sites using a single source of  φC31 recombinase. The development of modern genetic engineering […]

Y-chromosome transgenics using CRISPR/Cas9

Buchman et al (2018) from Tata Institute of California University reported in Insect Molecular Biology the use of CRISPR/Cas9-HDR system in order to insert transgenes to specific sites of the Drosophila melanogaster Y chromosome. In addition they investigated its usage […]

Switchable Genome Editing

  A recent manuscript by Suzuki et al (2018) demonstrates an effective method to control the expression of  CRISPR Cas9 based molecular components with a small molecule. Existing methods for controlling Cas9 suffer from high background activity and/or use compounds […]

An Enhanced CRISPR Repressor

Nan Cher Yeo and colleagues in the Church lab report in Nature Methods the identification of a combination of repressor domains which increases gene repression and could be widely useful. The CRISPR-Cas system is widely used as a powerful gene […]

Mitigating Resistance to Gene Drive

A recent manuscript posted on bioRiv by Hammond et al (2018) report exciting improvements to CRISPR-based suppression gene drives in Anopheles gambiae, with the development of new CRISPR designs that reduce selection of resistance and results in much higher suppression […]

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