Technology Topics

This is a community blog to capture ongoing developments in and applications of insect genetic technologies.
IGTRCN participants are encouraged to post to this blog when they find a Technology Topic of interest.
Use the Submissions button at the bottom of this page and select Technology Topics or comment directly on existing posts.
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Chemical and Light Inducible Recombinases

In a recent Nature Communications publication Weinberg et al., (2019) report developing and testing a repertoire of inducible site-specific recombinases capable of being activated by different inducers such as small molecules, light, and temperature.   While developed and tested in mammalian cells and transgenic mice, they will be widely useful in insect systems.

Gene Editing Screwworms and Blowflies

Paolo et al. report the first successful use of CRISPR/Cas9 in the New World Screwworm fly, Cochliomyia hominivorax (C. hominivorax), a parasite of warm blooded animals including humans, as well as a CRISPR/Cas9 protocol for the Australian sheep blowfly, Lucilia […]

“Sexing” Technologies – A Review

In a recent review,  Lutrat et al. (2019) provide a comprehensive summary of current (since 2003) sexing methods available for various dipteran pest species. The review distinguishes between early and late acting sexing methods and elaborates on the techniques used […]

CRISPR/Cas9-Mediated NHEJ Mutations in Anastrepha suspensa Paves Way for HDR

In an a recent experiment published in Gene, Li and Handler (2019) utilized readily available study systems and newly emerging genetic technologies and demonstrated both germline and somatic CRISPR/Cas9 induced mutations in Anastrepha ludens. The creation of heritable mutations was […]