ZFN

standard Loss of Function Mutations – Approaches, Limitations

Technologies are democratizing insect genetics and it seems as if everyone is thinking about making ‘knockouts’.  This is a good thing but as Housden et al. nicely explain in a recent paper in Nature Reveiws Genetics explain: “although LOF (loss […]

A selfish gene (blue) spreading through a mosquito population

standard Insect Gene Drives – Reviewed and Compared

Champer et al have published a review on the topic of engineered gene drives to manipulate wild populations in a recent Nature Reviews Genetics. Recent attention on Cas9-based gene drive systems has raised the awareness of these types of genetic […]

Danaus plexippus By William Warby (https://flic.kr/p/dtKZF2) [CC BY 2.0 (http://creativecommons.org/licenses/by/2.0)], via Wikimedia Commons

standard Monarch Reverse Genetics

Markert et al. just reported their effective use Cas9 and TALENs to knock out cryptochrome 2 and clock in Danaus plexippus using some strategies and approaches that are generally useful to insect scientists and much more efficient than earlier reports […]

standard Dopamine receptor knock-outs in crickets

Awata et al. (2015) have used Cas9 to knock-out Dop1 in Gryllus bimaculatus to assess the role of dopamine neurons in mediating reward and punishment. Functional genomics technologies like transposons and first and second generation gene-editing technologies have been available […]

standard Compendium of Genome Engineering

Koen Venken and colleagues have just published an excellent review of ‘Genome Engineering: Drosophila melanogaster and beyond‘ that is comprehensive and highly informative, and should serve as a useful reference for anyone interested in genome modification techniques with nucleotide precision […]

standard Powerful Genetic and Genomic Toolbox of Nasonia Wasps

Jeremy Lynch (2015) has published a nice overview of the genetic/genomic tools currently available for Nasonia species. Insects in the order of Hymenoptera are emerging as new genetic and genomic tools for evolutionary biology, development, and behavioral ecology. Among over […]

Cas9 + sgRNA

standard CRISPR-Cas9 Through Eyes of Two Leaders in the Field

In a comprehensive and definitive review published in Science, Jennifer Doudna and Emmanuelle Charpentier, two scientist who made seminal discoveries leading to the current boom in genome engineering, walk us through the (brief) history, scientific applications, and future promise of […]

Structure of a TALE binding to DNA

standard TAL Effector Modulators: Beyond Nucleases

Programmable DNA-binding proteins such as Zinc Finger Nucleases, TALEs and CRISPR/Cas9 are versatile tools for genome modification. While mutagenesis (knock outs and knock ins) has been a major initial objective in using these systems to date, it is clear that […]

standard Development, Growth & Differentiation Devote Issue to Genome Editing

Genome editing with engineered nucleases is an emerging technology that enables manipulation of targeted genes in many organisms and cell lines. Development, Growth & Differentiation has devoted an entire issue to genome editing.  The paper describing the successful use of […]