Vasa

Drosophila melanogaster

Cas9 Expression Patterns Impact on Resistance Evolution in Different Genetic Backgrounds

Gene drive systems are sensitive to the evolution of resistance in the form of polymorphisms in the guide RNA’s target DNA sequence.  Champer et al. (2017) have recently reported on their exploration of how Cas9 expression patterns as well as […]

Adult male spotted wing drosophila, Drosophilia suzukii (Matsumura). Photograph by Martin Hauser, California Department of Food and Agriculture.

Gene Editing in an Invasive Species

Li and Scott report their successful use of Cas9 to knockout two genes in Drosophila suzukii in a recent issue of Biochemical and Biophysical Research Communications. Drosophila suzukii or the spotted wing Drosophila although a native of Asia has recently […]

vasa Cas9 expressing Drosophila. From Xue et al. 2014

Vasa Cas9 Experession Yields Good Mutation Rates in Drosophila

Xue and colleagues report efficient knock-out and knock-in manipulations of the Drosophila genome with a Cas9 transgenic system. Others have made similar observations.  Xue et al. report the germline mutational rate ranging from 14% to 95%. They also demonstrated the […]