transgenics

Finding Enhancers in Mosquitoes and Beyond

This post was written by Joseph Sarro and Keshava Mysore In a recent paper published in BMC Genomics, Behura and Sarro et al. (2016) report on the use of FAIRE-seq, formaldehyde-assisted isolation of regulatory elements paired with DNA sequencing, for […]

Powerful Genetic and Genomic Toolbox of Nasonia Wasps

Jeremy Lynch (2015) has published a nice overview of the genetic/genomic tools currently available for Nasonia species. Insects in the order of Hymenoptera are emerging as new genetic and genomic tools for evolutionary biology, development, and behavioral ecology. Among over […]

International Short Course on Insect Transgenesis: Shanghai, China March 16-20, 2015

International Short Course on Insect Transgenesis. (download poster) Applications are now being accepted. The workshop will provide a small number (~15) of rigorously-selected graduate students, postdocs and early-stage faculty an opportunity to learn how to plan and carry out transgenesis […]

Bombyx mori with eggs

Transgenic TALEN Expression to Control Sex in Bombyx

Analysis of gene function in insects by editing the genome, or reverse genetics, is now greatly facilitated thanks to powerful genetic technologies, such as TALENs, ZFNs, and more recently CRISPR/Cas9, becoming available in many organisms. However, screening of transgenic mutated […]

Synthetic Selfish DNA as Genetic Technologies

  Selfish genes are genes that can spread themselves rapidly within genomes and populations.   Think – transposable elements, B chromosomes, homing endonucleases, selfish sex chromosomes, genomic exclusion to name a few. Some are promising candidates for the genetic engineering […]

Genetic Technology Delivery by Embryo Microinjection

Robotic Injection of Embryos – Ready for Insects?

  Spaink et al (2013) describe their use of a robotic injection system as a high throughput screening tool in zebra fish, Danio rerio, embryos.  Automated and semi-automated injection systems for zebra fish embryos as well as other systems are […]

Mutagenesis, Transposons and Transgenes: State-of-the-Art

The genetic tools available for use in Drosophila melanogaster are second to none. Insect scientists working on other insect systems and who also want to acquire or develop various genetic technologies should always be keeping their eyes on technological developments […]

XOR Circuit: You get output only when there is input from A or B but not both or neither.

CRISPy Circuits

Sophisticated tools for genome modification allow investigators to devise ever-increasing complex genetic circuits. The Gal4 /UAS system is one example of a simple binary system. While powerful, this system does not allow for particularly complex genetic circuits to be assembled. […]

Anopheles gambiae

Controlling Malaria Mosquitoes With Genetics

A chemical-free eradication method for the human malaria mosquito, Anopheles gambiae, that is self-sustaining and requires no additional inputs beyond ‘pushing the button’ to get it started, sounds fantastic. For geneticists it has not been terribly difficult to think of […]