transgenic

Basics of Cas9 Activity

standard Guide to Moving Genetic Technologies into Insects

Huang et al (2016) have published a short review on insect genetic technologies in the Journal of Genetics and Genomics that could serve as a useful guide for those considering the development of genetic technologies for ‘their’ insect system. The […]

standard Promoters From Two Sap-Sucking Pests

Qian et al (2016) using bioinformatic and transient heterologous expression systems have identified three strong promoters from the genomes of the Hemipterans  Nilaparvata lugens and Nephotettix cincticeps. The abundance of genome sequence data is a boon to insect science and […]

standard Animal and Insect Transgenics – Parallels

James Murray and Elizabeth Maga recently recounted briefly the development and evolution of animal transgenic technologies in a recent issue of Transgenic Research and the similarities and differences between the development and evolution of insect transgenic technologies, which were ongoing […]

standard Compendium of Genome Engineering

Koen Venken and colleagues have just published an excellent review of ‘Genome Engineering: Drosophila melanogaster and beyond‘ that is comprehensive and highly informative, and should serve as a useful reference for anyone interested in genome modification techniques with nucleotide precision […]

RNAi as an insecticide.

standard Insecticidal miRNA: Alternative to dsRNA and siRNA

RNA interference (RNAi) technology has been widely proposed to control insect pests by targeting their vital genes. Small interfering RNA (siRNA)-assisted RNAi has been reported in several previous studies. However, Agrawal et al 2015 report microRNA-assisted plant-mediated RNAi to control […]

Electroporation Theory

standard Electroporating the CRISPR/Cas9 System

Qin et al. (2015) describe their successful efforts to use electroporation technology to introduce CRISPR/Cas9 system components into mouse embryos and while not exactly insect biology their motivations for pursuing this approach are shared by many insect biologists with an interest […]

standard CRISPR with Independent Transgenes or Mutagenic Chain Reaction for Creating Homozygotes in Lab?

In fall of 2014, two publications came from George Church’s group (Esvelt et al., 2014; Oye et al., 2014) postulating on the potential for the CRISPR/Cas9 system to be used as an autonomous gene drive for various pest management goals. […]

standard Short Course on Insect Genetic Technologies – August 2015

The Insect Genetic Technologies Research Coordination Network is offering a short course on insect genetic technologies August 17-21, 2015. Today there are myriad technologies for modifying genomes. Technologies are emerging rapidly and it is a challenge for investigators to keep […]

standard Anopheles gambiae Transgenesis Tool Box – Major Additions

Volohonsky et. al. (2015) in a just-published paper in G3 entitled  “Tools for Anopheles gambiae transgenesis”  describe a wealth of new tools for creating transgenic An. gambiae. There has been a protocol for Anopheles gambiae transgenesis for over 15 years, […]

Electroporation Theory

standard Electroporation of Horn Fly Embryos

Qiang Xu, et al. 2015 describe their efforts to transform the horn fly, Haematobia irritans, by elctroporation. Transgenic technology has been considered an effective technique for the construction of genetically-modified insects for the purposes of insect control. Microinjection with piggyBac transposable […]

FlyFacility, Cambridge University. A insect genetic technologies service provider and resource

standard Fly Facility, University of Cambridge, UK

Insect Genetic Technologies Service Provider – Drosophila – Non-commercial Fly Facility, University of Cambridge, UK – http://www.flyfacility.gen.cam.ac.uk Our Drosophila microinjection service is available to fly researchers throughout the European Union. We can inject flies (including non-melanogaster Drosophila species) for P-element/piggyback […]

Simple representation of a 2-component system.

standard Borrowing Signaling Pathways from Bacteria for Use in Eukaryotes

Using genetic technologies for constructing signaling pathways in vivo opens the door to sophisticated manipulations of the host organism and Hansen et al (2014) in a recent issue of PNAS describe their successful transplantation of three two-component signaling pathways from […]

standard IGTRCN Trainees – Fall 2014!

The IGTRCN is working to diffuse knowledge and technology from those who ‘know’ and ‘have’ to those who do not but need to. The Peer-to-Peer Training Fellowships are intended to facilitate the process by providing modest resources to encourage interactions. […]

standard “Trojan Horse” Peptides: Delivery Systems With Diverse Applications

Everyone wants to get things into cells. Biomedical researchers, clinicians and even insect biologists. Consequently there is a large research community – primarily in the realm of biomedicine – that is actively exploring “delivery systems”. Cell Penetrating Peptides (CPPs), sometimes […]

Electroporation Theory

standard Injectoporation for Gene Delivery

Let’s see.  What do we have so far? Great genomic resources?  Check. Great gene vectors?  Check. Great genome modification technologies?  Check. Effective insect genetic technology delivery systems for somatic and germ cells?  Not so much, unfortunately. Gene, transgene and RNA […]

Medusa of Greek Mythology

standard Medusa: Harnessing a Sex-Linked Transgenic Drive System to Control Insect Populations

Segregation distorters (also known as drive genes) are selfish genetic elements with a natural fitness advantage – they are passed to greater than fifty percent of offspring in the next generation, breaking Mendel’s first law of inheritance. In nature, drive […]

standard Insect Genetic Technologies Service Providers

Insect Genetic Technology Service Providers If you would like to add to this list use the “Make a Submission” button and select “Knowledgebase” For insects other than Drosophila melanogaster Non-Commercial The Institute for Bioscience and Biotechnology Insect Transformation Facility, University […]

Everyone against Dengue

standard How to Release Genetically Modified Mosquitoes – The Right Way

Insect genetic technologies are enabling researchers to conceive of new ways to control insects and to improve on established control methods. In mosquitoes there have been a number of ideas for using genetic technologies to reduce mosquito populations or to […]

Meiotic drive in Drosophila melanogaster

standard The Road Ahead for Using Gene Drive Systems

Emerging technologies have employed chemical, ecological and genetic strategies for effective and economical pest management. The various strategies around the transgenic augmentation of pests has shown great promise in being able to combat vector borne diseases and eradicate agricultural pests […]

WHO Logo

standard WHO’s Guidance on Testing Genetically Modified Mosquitoes

The World Health Organization (WHO)  has published a guidance document that provides a framework by which genetically modified mosquitoes might be developed and tested as tools for controlling mosquito populations and/or pathogen transmission. This guidance comes at a time when […]

standard Six Tandem Cherries -> Very Bright Insects

Insect genetic modification technologies rely heavily on the use of fluorescent proteins as genetic markers, expression reporters etc. Colors vary, as do fluorescent outputs. Shearin et al. (2014) describe their efforts to increase signals from two popular fluorescent proteins, EGFP […]

TALENs and CRISPRs

standard Multigene Mutagenesis of Silk Moth with CRISPRs.

Genome editing using CRISPR/Cas9 is changing the way we think about genome modification. The CRISPR system, because it relies on a small user-provide guide RNA (gRNA) to give the Cas9 endonuclease its sequence specificity, is easy to use, relative to, […]

Sheep Blow Fly, Lucilia cuprina

standard Insect Genetic Technologies To Control Blow Flies

Blow flies (family Calliphoridae) can be serious and dangerous pests. The new world screwworm fly (Cochliomyia hominivorax) as the latin name says can be a man-eater (hominivorax). While many blow flies prefer to lay eggs on dead and decaying animals, […]

I-CreI Crystal Structure

standard Delivering Reprogramed Meganucleases With Lentiviruses- Is this Applicable to “Difficult” Insects?

Delivering biologically relevant nucleic acids and proteins to appropriate cells of multicellular organisms for the purposes of genetically modifying either somatic or germline genomes is a central problem for not only insect biologists but for those working on other organisms […]

Platynereis dumerilii adult

standard Genetically Modified Annelids: Platynerieis dumerilii. Not Unlike Modifying Insects

The bristle worm, Platynerieis dumerilii, is a marine annelid with a biology that makes it of interest because of the development of its nervous system, reproductive cycles that are synchronized with lunar phases and its neuroendocrinology among other things. Even […]

Apis mellifera

standard Transgenic Honeybees – Finally!

Genome modification technologies have been increasing steadily in recent years. With the exception of direct genome editing technologies (ZFNs, TALENs and CRISPRs) variously technologies for use in insects rely heavily on transposon platforms to move these technologies into target genomes. […]

Daphnia pulex

standard Water Fleas and Insects – Common Challenges for Genetic Modification – Shared Solutions?

The water flea, Daphnia pulex, is a microcrustacean that has become a model for biomedical research and, perhaps more significantly, a model for probing G x E (Genetics x Environment) interactions. Some genetic modification methods have been developed for Daphnia […]

Strategy for Y engineering; from Bernardini et al. 2014

standard Advanced Mosquito Genetic Engineering

For mosquitoes the number of genetic modification technologies that can and have been applied continues to grow, and while still not as deep as the Drosophila toolbox the mosquito toolbox is still impressive in the right hands. Bernardini et al. […]

Chimera, a mythic creature composed of lion, snake and goat

standard Marking Clones: Past and Future

As Perrimon and colleagues point out chimeras or mosaics have played and continue to play an important role in our analysis of development.  In Drosophilia the technology for creating mosaics is mature and powerful.  Even before transgenic technologies provided precision […]

Insect Genetic Technologies for Mosquito Control

standard Applying Insect Genetic Technologies to Mosquito Control

The development and use of genetic technologies in a few mosquito species that are major vectors of human pathogens has been on factor in driving or, more appropriately, reviving interest in using genetics-based strategies to control these insects. Transgenic technologies […]