transformation

Genetic Technology Delivery by Embryo Microinjection

standard IGTRCN Technical Course 1.0 a Great Success!

Beginning on August 17, 2015 25 scientists from laboratories of 9 countries were immersed in the theory and practice of insect genetic technologies and left at the end of the week exhausted but well trained and with a solid foundation […]

standard Megaselia and Chironomus Germline Transformation

In a recent paper in Development Genes Evolution., Caroti et al. (2015) reported on their efforts to extend the piggyBac transposon-based germline transformation system to the Diptera Megaselia abdita and Chironomus riparius and to use it for in vivo labelling […]

standard Short Course on Insect Genetic Technologies – August 2015

The Insect Genetic Technologies Research Coordination Network is offering a short course on insect genetic technologies August 17-21, 2015. Today there are myriad technologies for modifying genomes. Technologies are emerging rapidly and it is a challenge for investigators to keep […]

Electroporation Theory

standard Electroporation of Horn Fly Embryos

Qiang Xu, et al. 2015 describe their efforts to transform the horn fly, Haematobia irritans, by elctroporation. Transgenic technology has been considered an effective technique for the construction of genetically-modified insects for the purposes of insect control. Microinjection with piggyBac transposable […]

standard Delivering gene-editing proteins in vivo using a new strategy.

Delivering genetic technologies to appropriate cells at the appropriate times is, for many insect systems, the biggest obstacle to the widespread use of these technologies. Microinjecting developing embryos with DNA, RNA or protein remains the dominant mode of delivery in […]

Electroporation Theory

standard Injectoporation for Gene Delivery

Let’s see.  What do we have so far? Great genomic resources?  Check. Great gene vectors?  Check. Great genome modification technologies?  Check. Effective insect genetic technology delivery systems for somatic and germ cells?  Not so much, unfortunately. Gene, transgene and RNA […]

standard φC31 in Anopheles gambiae: A How-To

In terms of genetic engineering of insects, the current most commonly used technique for germline transformation involves the use of mobile transposable genetic elements, which are simply inefficient, have limited carrying capacities, and integrate randomly into genomes resulting in insertional […]

Genetic Technology Delivery by Embryo Microinjection

standard Robotic Injection of Embryos – Ready for Insects?

  Spaink et al (2013) describe their use of a robotic injection system as a high throughput screening tool in zebra fish, Danio rerio, embryos.  Automated and semi-automated injection systems for zebra fish embryos as well as other systems are […]