TALENs

Danaus plexippus By William Warby (https://flic.kr/p/dtKZF2) [CC BY 2.0 (http://creativecommons.org/licenses/by/2.0)], via Wikimedia Commons

standard Monarch Reverse Genetics

Markert et al. just reported their effective use Cas9 and TALENs to knock out cryptochrome 2 and clock in Danaus plexippus using some strategies and approaches that are generally useful to insect scientists and much more efficient than earlier reports […]

standard Powerful Genetic and Genomic Toolbox of Nasonia Wasps

Jeremy Lynch (2015) has published a nice overview of the genetic/genomic tools currently available for Nasonia species. Insects in the order of Hymenoptera are emerging as new genetic and genomic tools for evolutionary biology, development, and behavioral ecology. Among over […]

standard Comparing TALE- and dCas9-Based Designer Transcription Factors

Genome editing with programmable nucleases using transcription activator-like effector nucleases (TALENs) or RNA-guided clustered regularly interspersed short palindromic repeat (CRISPR) associated protein (Cas9) have become popular and important technologies. These systems can be modified to carry other functional protein domains […]

standard A Collection of Methods for Editing Insect Genomes

The August 2014 issue of METHODS is devoted to the topic of “Editing and Investigating Genomes with TALE and CRISPER/Cas Systems: Genome Engineering Across Species Using TALENS” Five of the 14 protocols included in this issue are specifically focused on […]

Bombyx mori with eggs

standard Transgenic TALEN Expression to Control Sex in Bombyx

Analysis of gene function in insects by editing the genome, or reverse genetics, is now greatly facilitated thanks to powerful genetic technologies, such as TALENs, ZFNs, and more recently CRISPR/Cas9, becoming available in many organisms. However, screening of transgenic mutated […]

standard Synthetic Selfish DNA as Genetic Technologies

  Selfish genes are genes that can spread themselves rapidly within genomes and populations.   Think – transposable elements, B chromosomes, homing endonucleases, selfish sex chromosomes, genomic exclusion to name a few. Some are promising candidates for the genetic engineering […]

BuDN (Nuclease) and TALENs (from Stella et al. 2014)

standard BuD: Another Tunable DNA-Binding Domain for Genome Modification

Tunable DNA-binding domains with high specificity remain important tools for constructing genome modifications even with the current popularity of the CRISPR/Cas9 system. Stella at al (2014) describe their analysis of a DNA-binding protein, BurrH, from the symbiotic bacterium Burkholderia rhizoxinica […]

standard Development, Growth & Differentiation Devote Issue to Genome Editing

Genome editing with engineered nucleases is an emerging technology that enables manipulation of targeted genes in many organisms and cell lines. Development, Growth & Differentiation has devoted an entire issue to genome editing.  The paper describing the successful use of […]