TALEN

standard Loss of Function Mutations – Approaches, Limitations

Technologies are democratizing insect genetics and it seems as if everyone is thinking about making ‘knockouts’.  This is a good thing but as Housden et al. nicely explain in a recent paper in Nature Reveiws Genetics explain: “although LOF (loss […]

A selfish gene (blue) spreading through a mosquito population

standard Insect Gene Drives – Reviewed and Compared

Champer et al have published a review on the topic of engineered gene drives to manipulate wild populations in a recent Nature Reviews Genetics. Recent attention on Cas9-based gene drive systems has raised the awareness of these types of genetic […]

standard Dopamine receptor knock-outs in crickets

Awata et al. (2015) have used Cas9 to knock-out Dop1 in Gryllus bimaculatus to assess the role of dopamine neurons in mediating reward and punishment. Functional genomics technologies like transposons and first and second generation gene-editing technologies have been available […]

Harmonia axyridis, 19-spot form

standard Sawfly and Ladybird Beetle Gene Editing

Hatakeyama et al (2015) have successfully used gene editing technology in Athalia rosae (sawfly) and Harmonia axyridis (ladybird beetle), enlarging the range of insects in which these technologies have been applied.  These results are reported in Insect Molecular Biology. Hatakeyama […]

standard Compendium of Genome Engineering

Koen Venken and colleagues have just published an excellent review of ‘Genome Engineering: Drosophila melanogaster and beyond‘ that is comprehensive and highly informative, and should serve as a useful reference for anyone interested in genome modification techniques with nucleotide precision […]

Structure of a TALE binding to DNA

standard Optimized TALEN Application and Screening in Drosophila melanogaster

Lee et al 2015 provide a detailed methodology for gene editing using TALENs in Drosophila melanogaster. While TALEN use is relatively well established in D. melanogaster, fine tuning the injection concentrations of designer nucleases allows for the maximum efficiency in […]

Cas9 + sgRNA

standard CRISPR-Cas9 Through Eyes of Two Leaders in the Field

In a comprehensive and definitive review published in Science, Jennifer Doudna and Emmanuelle Charpentier, two scientist who made seminal discoveries leading to the current boom in genome engineering, walk us through the (brief) history, scientific applications, and future promise of […]

standard TGV: Programmable DNA Binding Proteins for Live Visualization of Chromatin

Transcription activator-like effector (TALE) technologies are being used in a variety of creative ways by swapping functional protein domains that are connected to the ‘programmed’ DNA binding domain.  Miyanari (2014) and Miyanari et al (2013) describe their use of TALEs […]

standard “CHOPCHOP”ing with CRISPRs and TALENs: A New Web Resource for Genome Editing Design

With the growing influence and applicability of genome editing technologies, TALENs and more recently CRISPRs, offer genetic capabilities to an ever growing list of organisms. In turn, as more and more researchers become interested in using these technologies, a common […]

Structure of a TALE binding to DNA

standard TAL Effector Modulators: Beyond Nucleases

Programmable DNA-binding proteins such as Zinc Finger Nucleases, TALEs and CRISPR/Cas9 are versatile tools for genome modification. While mutagenesis (knock outs and knock ins) has been a major initial objective in using these systems to date, it is clear that […]

BuDN (Nuclease) and TALENs (from Stella et al. 2014)

standard BuD: Another Tunable DNA-Binding Domain for Genome Modification

Tunable DNA-binding domains with high specificity remain important tools for constructing genome modifications even with the current popularity of the CRISPR/Cas9 system. Stella at al (2014) describe their analysis of a DNA-binding protein, BurrH, from the symbiotic bacterium Burkholderia rhizoxinica […]