TALEN

Loss of Function Mutations – Approaches, Limitations

Technologies are democratizing insect genetics and it seems as if everyone is thinking about making ‘knockouts’.  This is a good thing but as Housden et al. nicely explain in a recent paper in Nature Reveiws Genetics explain: “although LOF (loss […]

A selfish gene (blue) spreading through a mosquito population

Insect Gene Drives – Reviewed and Compared

Champer et al have published a review on the topic of engineered gene drives to manipulate wild populations in a recent Nature Reviews Genetics. Recent attention on Cas9-based gene drive systems has raised the awareness of these types of genetic […]

Harmonia axyridis, 19-spot form

Sawfly and Ladybird Beetle Gene Editing

Hatakeyama et al (2015) have successfully used gene editing technology in Athalia rosae (sawfly) and Harmonia axyridis (ladybird beetle), enlarging the range of insects in which these technologies have been applied.  These results are reported in Insect Molecular Biology. Hatakeyama […]

Structure of a TALE binding to DNA

Optimized TALEN Application and Screening in Drosophila melanogaster

Lee et al 2015 provide a detailed methodology for gene editing using TALENs in Drosophila melanogaster. While TALEN use is relatively well established in D. melanogaster, fine tuning the injection concentrations of designer nucleases allows for the maximum efficiency in […]

“CHOPCHOP”ing with CRISPRs and TALENs: A New Web Resource for Genome Editing Design

With the growing influence and applicability of genome editing technologies, TALENs and more recently CRISPRs, offer genetic capabilities to an ever growing list of organisms. In turn, as more and more researchers become interested in using these technologies, a common […]

BuDN (Nuclease) and TALENs (from Stella et al. 2014)

BuD: Another Tunable DNA-Binding Domain for Genome Modification

Tunable DNA-binding domains with high specificity remain important tools for constructing genome modifications even with the current popularity of the CRISPR/Cas9 system. Stella at al (2014) describe their analysis of a DNA-binding protein, BurrH, from the symbiotic bacterium Burkholderia rhizoxinica […]