Streptococcus

Guide RNA interacting with target DNA. Image credit: William Reid

Guide-RNA Engineering – Enhancing Activity and Function

The functionality of Cas9 is determined in part by the guide RNA with which it is associated. Norwak et al.(2016) review briefly how guide RNAs can be modified to enhance their function and to functionalize them in novel ways. Norwak […]

CRISPR/Cas9 Knock-in Technology

Cas9 Against RNA Targets In Vivo

Price et al (2015) have recently published a paper in PNAS in which they report that Cas9 can be used as a RNA-targeting system. Clustered, regularly interspersed, short palindromic repeats-CRISPR associated (CRISPR-Cas) systems are a diverse collection of adaptive immune […]