siRNA

standard Nanoparticle-bound, Aerosolized siRNA Knockdown in Aphids

Thairu et al. (2017) report in Insect Molecular Biology their use of aerosolized siRNAs bound to nanoparticles to silence genes in the pea aphid, Acyrthosiphon pisum, while circumventing  the problem of rapid dsRNA degradation in the hemolymph of this species. […]

standard RNAi-based therapeutics: Delivery is key and relevant to insects

Specific gene knockdown after exogenous small interfering RNA (siRNA) injection into humans and mammals holds great promise for therapeutic applications. But enthusiasm  waned and some predict its demise because of the manifold problems in delivering the technology. A new review […]

standard Tripodal RNA: An Enhanced RNAi Trigger

RNA interference-based manipulation of gene expression is a highly versatile tool available to insect scientists.  In some cases direct injection of large amounts of long dsRNA will suffice.  In other cases, not so much.  Combining siRNA with some kind of […]

Basic structure of a morpholino oligonucleotide

standard Have You Considered Morpholinos?

Many are making efforts to move genetic technologies into insect systems where they have not been used before  and becoming painfully aware that some technologies may not be applicable to all insects. For example, dsRNA may work well when systemically […]