RNAi

standard Nanoparticle-bound, Aerosolized siRNA Knockdown in Aphids

Thairu et al. (2017) report in Insect Molecular Biology their use of aerosolized siRNAs bound to nanoparticles to silence genes in the pea aphid, Acyrthosiphon pisum, while circumventing  the problem of rapid dsRNA degradation in the hemolymph of this species. […]

standard Genetic Dissection of RNAi in the Colorado Potato Beetle, Leptinotarsa decemlineata

RNAi works well in beetles, in general, and why that is is not all that clear but Yoon et al. (2016) have begun to identify key genetic contributors in their recent paper in Insect Biochemistry and Molecular Biology.  The authors […]

on. Image credit: www.biocomicals.com by Alper Uzun, PhD

standard RNAi in Beetles and Leps – Why the Difference?

It is well established that the RNAi response of Coleoptera and Lepidoptera differ greatly but less is known about the basis for these differences.  Skukla et al (2016) systematically investigated this question and report their insights in a recent paper […]

standard Post-feeding and -injection Dynamics of dsRNA in 4 Insects

The variability of dsRNA-induced RNA interference is well documented with some insects very amenable to this method of regulating gene expression while are others are very much less so. The factors involved in this variation are known to some extent […]

standard Lipoplexes and oral dsRNA delivery to Blattella germanica

Lin et al., in Pesticide Management Science have described their efforts to orally deliver dsRNA to the German cockroach, Blattella germanica. Blattella germanica is fairly sensitive to RNAi when dsRNA is injected into the hemocoel.  Directly feeding B. germanica dsRNA […]

standard Carbon Quantum Dots for dsRNA Delivery

Das et al. (2015) in Applied Materials & Interfaces compare chitosan, silica and carbon quantum dot nanoparticles as dsRNA delivery platforms for silencing genes in larval mosquitoes. Direct injection of dsRNA into the hemocoel of insects has  been somewhat effective […]

standard Extreme Transgenerational Gene Silencing

Abdellatef et al. (2015) recently published the results of their efforts to silence the salivary sheath protein gene in the aphid Sitobion avenae in the Plant Biotechnology Journal and observed what might be called extreme transgenerational gene silencing. Plant-expressed dsRNA […]

standard Parental RNAi Via Oral Delivery

Khajuria et al (2015) have published a short paper in Insect Biochemistry and Molecular Biology describing their observations supporting the idea that Diabrotica vigifera exhibits parental RNAi following the oral delivery of dsRNA. Parental RNAi (pRNAi) refers to an inter-generational […]

RNAi as an insecticide.

standard Insecticidal miRNA: Alternative to dsRNA and siRNA

RNA interference (RNAi) technology has been widely proposed to control insect pests by targeting their vital genes. Small interfering RNA (siRNA)-assisted RNAi has been reported in several previous studies. However, Agrawal et al 2015 report microRNA-assisted plant-mediated RNAi to control […]

standard Powerful Genetic and Genomic Toolbox of Nasonia Wasps

Jeremy Lynch (2015) has published a nice overview of the genetic/genomic tools currently available for Nasonia species. Insects in the order of Hymenoptera are emerging as new genetic and genomic tools for evolutionary biology, development, and behavioral ecology. Among over […]

standard Plastid Mediated RNAi: A Novel Insecticidal Tool

Recently, Zhang et al demonstrated a robust and promising way of controlling the devastating solanaceous crops insect pest, Colorado potato beetle (Leptinotarsa decemlineata; CPB), using a modified strategy for delivering plant mediated RNAi. Increasing insect herbivores’ resistance to plant defense mechanisms […]

standard RNAi-based therapeutics: Delivery is key and relevant to insects

Specific gene knockdown after exogenous small interfering RNA (siRNA) injection into humans and mammals holds great promise for therapeutic applications. But enthusiasm  waned and some predict its demise because of the manifold problems in delivering the technology. A new review […]

standard The Drosophila RNAi Screening Center (DRSC) – A functional genomics resource useful to all insect biologists.

The Drosophila RNAi Screening Center (DRSC) at Harvard Medical School was founded by Prof. Norbert Perrimon in 2003 to provide community support for genome-wide RNAi screens in cultured Drosophila cells. Now, more than a decade later, we are still going […]

standard Tripodal RNA: An Enhanced RNAi Trigger

RNA interference-based manipulation of gene expression is a highly versatile tool available to insect scientists.  In some cases direct injection of large amounts of long dsRNA will suffice.  In other cases, not so much.  Combining siRNA with some kind of […]

standard IGTRCN Trainees – Fall 2014!

The IGTRCN is working to diffuse knowledge and technology from those who ‘know’ and ‘have’ to those who do not but need to. The Peer-to-Peer Training Fellowships are intended to facilitate the process by providing modest resources to encourage interactions. […]

standard “Trojan Horse” Peptides: Delivery Systems With Diverse Applications

Everyone wants to get things into cells. Biomedical researchers, clinicians and even insect biologists. Consequently there is a large research community – primarily in the realm of biomedicine – that is actively exploring “delivery systems”. Cell Penetrating Peptides (CPPs), sometimes […]

Basic structure of a morpholino oligonucleotide

standard Have You Considered Morpholinos?

Many are making efforts to move genetic technologies into insect systems where they have not been used before  and becoming painfully aware that some technologies may not be applicable to all insects. For example, dsRNA may work well when systemically […]

Feeding Pea Aphid, Acyrthosiphon pisum

standard RNA Silencing in Plants to Fight Aphids

Aphids feed from the plant phloem sap and are responsible for large damage on crops by either weakening the plant or transmitting plant viruses that circulate in the sap. Can we use transgenic plants to limit aphid negative impact on […]

Culex quinquifasciatus pupa

standard dsRNA into Pupae for Adult Silencing – Mosquitoes

Although injection of large volumes and amounts of dsRNA into insects is somewhat of a blunt instrument for performing reverse genetics, it has been used with great success in many systems while in others it has been less useful. Stage […]

Daphnia pulex

standard Water Fleas and Insects – Common Challenges for Genetic Modification – Shared Solutions?

The water flea, Daphnia pulex, is a microcrustacean that has become a model for biomedical research and, perhaps more significantly, a model for probing G x E (Genetics x Environment) interactions. Some genetic modification methods have been developed for Daphnia […]

standard Feed or Inject dsRNA in Aphids – It Depends

RNAi-based gene silencing has been reported but it has been problematic. The authors’ direct comparison of injection and feeding of dsRNA targeting the cathepsin-L gene in the pea aphid, Acyrthosiphon pisum. Single aphids were analyzed at the level of five […]

Chimera, a mythic creature composed of lion, snake and goat

standard Marking Clones: Past and Future

As Perrimon and colleagues point out chimeras or mosaics have played and continue to play an important role in our analysis of development.  In Drosophilia the technology for creating mosaics is mature and powerful.  Even before transgenic technologies provided precision […]

Feeding or Microinjecting dsRNA into Aphids for Gene Silencing.

standard Aphid Hemolymph and Gut Degrade dsRNA

Aphids are sap-sucking insects of great economic importance, and recently the pea aphid Acyrthosiphon pisum had its genome sequenced and annotated.  Insect genetic technologies are in high demand for this insect but it is proving to be a real challenge. […]