piggyBac

standard Western Corn Rootworm, Diabrotica virgifera virgifera, germline transformation.

Chu and colleagues in a recently published paper in Insect Molecular Biology describe their successful efforts to deploy transposon-based technologies in the economically important beetle, Diabrotica virgifera virgifera, and it is an interesting case study in how to develop genetic […]

standard Transgenic blow flies for SIT

Yan and Scott have constructed transgenic Lucilia cuprina, Australian sheep blowfly, resulting in conditional lethality of female embryos and their results are reported in Scientific Reports. With an interest in enabling the development of efficient systems for producing male blowflies […]

standard Glycoengineering Silkworms Using Transposon Technology

Mabashi-Asazuma et al. (2015)  report in Insect Biochemistry and Molecular Biology on their successful efforts to alter post translational glycolytic processing of proteins in the posterior silk gland of Bombyx mori. The “silkworm is emerging as a new recombinant protein […]

standard Megaselia and Chironomus Germline Transformation

In a recent paper in Development Genes Evolution., Caroti et al. (2015) reported on their efforts to extend the piggyBac transposon-based germline transformation system to the Diptera Megaselia abdita and Chironomus riparius and to use it for in vivo labelling […]

standard Anopheles gambiae Transgenesis Tool Box – Major Additions

Volohonsky et. al. (2015) in a just-published paper in G3 entitled  “Tools for Anopheles gambiae transgenesis”  describe a wealth of new tools for creating transgenic An. gambiae. There has been a protocol for Anopheles gambiae transgenesis for over 15 years, […]

Bombyx mori with eggs

standard A Stable, Replaceable, Highly Efficient Transgene Expression System

Long et. al. in Scientific Reports describe a system for creating stable sites in the genome of Bombyx mori that allow for site-specific recombination-mediated integration of transgenes. Transposons are essential platforms for many insect genetic technologies and are typically used […]

standard New ΦC31 Resources

A rapid method for generating and finding ΦC31 landing sites (attP) with the desired expression characteristics as well as a new method for creating transgene arrays in vivo was recently described by Knapp et al (2015). Albeit these methods were […]

Electroporation Theory

standard Electroporation of Horn Fly Embryos

Qiang Xu, et al. 2015 describe their efforts to transform the horn fly, Haematobia irritans, by elctroporation. Transgenic technology has been considered an effective technique for the construction of genetically-modified insects for the purposes of insect control. Microinjection with piggyBac transposable […]

standard Two Bombyx Testis-Specific Promoters

The level of sophistication with which one can genetically manipulate insects is very much determined by how well transgene expression can be regulated. Xu et al (2014) in Insect Molecular Biology describe their successful efforts to isolate promoters from two […]

Bombyx mori with eggs

standard Transgenic TALEN Expression to Control Sex in Bombyx

Analysis of gene function in insects by editing the genome, or reverse genetics, is now greatly facilitated thanks to powerful genetic technologies, such as TALENs, ZFNs, and more recently CRISPR/Cas9, becoming available in many organisms. However, screening of transgenic mutated […]

standard Synthetic Selfish DNA as Genetic Technologies

  Selfish genes are genes that can spread themselves rapidly within genomes and populations.   Think – transposable elements, B chromosomes, homing endonucleases, selfish sex chromosomes, genomic exclusion to name a few. Some are promising candidates for the genetic engineering […]

Harmonia axyridis, 19-spot form

standard Enhancer Trapping in the Ladybeetle, Harmonia axyridis

Nowadays, efforts to sequence genomes are routine experiments. But once it comes down to the bioinformatics and especially to understand the vast amount of data on a functional level – we are still struggling and good old techniques to find […]

Sheep Blow Fly, Lucilia cuprina

standard Insect Genetic Technologies To Control Blow Flies

Blow flies (family Calliphoridae) can be serious and dangerous pests. The new world screwworm fly (Cochliomyia hominivorax) as the latin name says can be a man-eater (hominivorax). While many blow flies prefer to lay eggs on dead and decaying animals, […]

Apis mellifera

standard Transgenic Honeybees – Finally!

Genome modification technologies have been increasing steadily in recent years. With the exception of direct genome editing technologies (ZFNs, TALENs and CRISPRs) variously technologies for use in insects rely heavily on transposon platforms to move these technologies into target genomes. […]

standard What a TRIP!

Anyone ever making or working with transgenics constructed with transposon vectors has experienced “position effects”. Position effects refers to variation in transgene expression as a function of integration site location. This can make comparisons of different transgenes in different locations […]

Strategy for Y engineering; from Bernardini et al. 2014

standard Advanced Mosquito Genetic Engineering

For mosquitoes the number of genetic modification technologies that can and have been applied continues to grow, and while still not as deep as the Drosophila toolbox the mosquito toolbox is still impressive in the right hands. Bernardini et al. […]

Microinjection needles and life stages of four species of Lepidoptera. from Beaudette et al. 2014, Biotechniques

standard Germ-Line Transformation of Junonia coenia

Many of today’s insect genetic technologies are delivered to the germ-line of target species by direct injection of developing embryos. This is actually a major limitation to the adoption of these powerful technologies in some insect research communities– one simply […]