off-target

Cas9 + sgRNA

CRISPRscan – A Tool Aiding Selection of CRISPR-Cas9 gRNAs

Just published online in Nature Methods, Moreno-Mateos et al report testing the activities of >1200 gRNAs in zebrafish embryos and used the results to create an algorithm for predicting gRNA efficacy. The advent of the CRISPR-Cas9 system has revolutionized genome […]

“CHOPCHOP”ing with CRISPRs and TALENs: A New Web Resource for Genome Editing Design

With the growing influence and applicability of genome editing technologies, TALENs and more recently CRISPRs, offer genetic capabilities to an ever growing list of organisms. In turn, as more and more researchers become interested in using these technologies, a common […]