NHEJ

A recent scheme showing the consequences of distorting mosquito sex ratios using a "precision" drive system.

Strategies for Driving Out Invasive Species

Prowse et al. (2017) present their modelling work in the Proceedings of The Royal Society B on various different CRISPR/Cas9-based gene drives aimed at suppressing populations of exotic vertebrate species on islands (focusing on mice (Mus musculus)).  The issues addressed […]

Drosophila melanogaster

Cas9 Expression Patterns Impact on Resistance Evolution in Different Genetic Backgrounds

Gene drive systems are sensitive to the evolution of resistance in the form of polymorphisms in the guide RNA’s target DNA sequence.  Champer et al. (2017) have recently reported on their exploration of how Cas9 expression patterns as well as […]

Cas9 with functional domains highlighted in relation to target DNA and guideRNA

RAD51 and Valporic Acid Enhance Cas9-induced HR

Takayama et al (2017) describe in Nucleic Acids Research how they increased the frequency of biallelic editing of genes in human ES/iPS cells and their findings may be useful to insect biologists. The efficiency of Cas9-mediated homologous recombination (HR) can […]