NHEJ

Drosophila melanogaster

standard Cas9 Expression Patterns Impact on Resistance Evolution in Different Genetic Backgrounds

Gene drive systems are sensitive to the evolution of resistance in the form of polymorphisms in the guide RNA’s target DNA sequence.  Champer et al. (2017) have recently reported on their exploration of how Cas9 expression patterns as well as […]

User-designed molecular scissors.

standard Dynamics of Gene Drive Resistance – Lab Cage Trials

In their current pre-publication Hammond et al investigate the emergence of resistance to a homing-based gene drive designed to disrupt a gene essential for female Anopheles gambiae fertility. In recent years, there has been much theoretical design and modeling work […]

Cas9 with functional domains highlighted in relation to target DNA and guideRNA

standard RAD51 and Valporic Acid Enhance Cas9-induced HR

Takayama et al (2017) describe in Nucleic Acids Research how they increased the frequency of biallelic editing of genes in human ES/iPS cells and their findings may be useful to insect biologists. The efficiency of Cas9-mediated homologous recombination (HR) can […]

standard Dominant Maternal Effects Caused by CRISPR/Cas9

Expressing Cas9 and guide RNAs from transgenes integrated into the genome of organisms typically leads to higher rates of mutagenesis of the target gene compared to when those components are provided transiently by microinjection, for example. Lin and Potter describe […]

standard CRISPR with Independent Transgenes or Mutagenic Chain Reaction for Creating Homozygotes in Lab?

In fall of 2014, two publications came from George Church’s group (Esvelt et al., 2014; Oye et al., 2014) postulating on the potential for the CRISPR/Cas9 system to be used as an autonomous gene drive for various pest management goals. […]