mouse

standard Microinjection independent genome engineering

Takahashi et al. (2105) describe a microinjection independent genome engineering method for mice that is an interesting innovation that seeks to address technical challenges associated with the creation of transgenic and genetically modified mice that have good analogs in insect […]

Electroporation Theory

standard Electroporating the CRISPR/Cas9 System

Qin et al. (2015) describe their successful efforts to use electroporation technology to introduce CRISPR/Cas9 system components into mouse embryos and while not exactly insect biology their motivations for pursuing this approach are shared by many insect biologists with an interest […]

standard What a TRIP!

Anyone ever making or working with transgenics constructed with transposon vectors has experienced “position effects”. Position effects refers to variation in transgene expression as a function of integration site location. This can make comparisons of different transgenes in different locations […]