mosquito

Crystal Structure of Cas9 bound to DNA based on the Anders et al 2014 Nature paper. Rendition was performed using UCSF's chimera software. Image used under Creative Commons License.

A Protocol for the Highly Efficient Generation of Stable Mosquito Knockout Lines Using CRISPR/Cas9

In their recent paper in G3: Genes|Genomes|Genetics, Li et al. describe a highly efficient protocol for the generation of stable knockout lines using CRISPR/Cas9. The ability of effectively targeting and disrupting gene function is essential for gene functional analysis studies […]

Synthetic Selfish DNA as Genetic Technologies

  Selfish genes are genes that can spread themselves rapidly within genomes and populations.   Think – transposable elements, B chromosomes, homing endonucleases, selfish sex chromosomes, genomic exclusion to name a few. Some are promising candidates for the genetic engineering […]

Genetic Technology Delivery by Embryo Microinjection

Robotic Injection of Embryos – Ready for Insects?

  Spaink et al (2013) describe their use of a robotic injection system as a high throughput screening tool in zebra fish, Danio rerio, embryos.  Automated and semi-automated injection systems for zebra fish embryos as well as other systems are […]

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WHO’s Guidance on Testing Genetically Modified Mosquitoes

The World Health Organization (WHO)  has published a guidance document that provides a framework by which genetically modified mosquitoes might be developed and tested as tools for controlling mosquito populations and/or pathogen transmission. This guidance comes at a time when […]